The total protein test is a rough measure of all the proteins found in the fluid portion of your blood. Specifically it looks at the total amount of two classes of proteins: albumin and globulin.

Proteins are important parts of all cells and tissues. For example, albumin helps maintain a certain type of blood pressure by preventing fluid from leaking out of blood vessels.

Blood is drawn from a vein or a capillary. The blood sample is placed into a machine called a centrifuge, which spins the blood to separate the serum from the cells. The total protein test is done on serum.

Your health care provider may tell you to stop taking certain drugs that can affect the test.

Drugs that can increase total protein measurements include anabolic steroids, androgens, corticosteroids, dextran, growth hormone, insulin, phenazopyridine, and progesterone.

Drugs that can decrease total protein measurements include ammonium ions, estrogens, hepatotoxic drugs, and oral contraceptives.

This test is often done to diagnose nutritional problems, kidney disease or liver disease. If total protein is abnormal, further tests must be done to identify the specific problem.

The normal range is 6.0 to 8.3 gm/dl (grams per deciliter).

Normal values may vary slightly from laboratory to laboratory.

Higher-than-normal levels may be due to:

• Chronic inflammation or infection, including HIV and hepatitis B or C
• Multiple myeloma
• Waldenstrom's disease

Lower-than-normal levels may be due to:

• Agammaglobulinemia
• Bleeding (hemorrhage)
• Burns (extensive)
• Glomerulonephritis
• Liver disease
• Malabsorption
• Malnutrition
• Protein-losing enteropathy

Additional conditions under which the test may be performed:

• Congenital nephrotic syndrome

Total protein measurement may be increased during pregnancy.