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polyclonal Ab against the variable region
by Pery, Jul 26, 2006 12:00AM
I need help please.
I want to produce polyclonal Ab against the part of the variable region (CDR1 mostly) of human monoclonal Ab (IgG).
In my previous trials I've injected mAb into the rabbit and cleaned the pAb on G-sepharose column. Eventually I’ve got the non specific pAb population i.e. it recognizes many common IgG determinants.
I have very little quantity of the mAb so I cannot slice it in order to rid of the common parts (like Fc for example).
Please advice if there are ways to produce determinant specific anti-Ab antibodies (poly or mono).
Thanks in advance.
Pery
I want to produce polyclonal Ab against the part of the variable region (CDR1 mostly) of human monoclonal Ab (IgG).
In my previous trials I've injected mAb into the rabbit and cleaned the pAb on G-sepharose column. Eventually I’ve got the non specific pAb population i.e. it recognizes many common IgG determinants.
I have very little quantity of the mAb so I cannot slice it in order to rid of the common parts (like Fc for example).
Please advice if there are ways to produce determinant specific anti-Ab antibodies (poly or mono).
Thanks in advance.
Pery
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