Member Comments are provided by individuals and reflect their personal opinions only. Under NO circumstances should you act on any advice or opinion posted in this forum. ALWAYS check with your personal physician before taking any action regarding your health! MedHelp International and our partners, sponsors and affiliates have no obligation to monitor any comments posted on this site, or the content and/or accuracy of such exchanges. MedHelp International does not endorse the views of any user.
polyclonal Ab against the variable region
by Pery, Jul 26, 2006 12:00AM
I need help please.
I want to produce polyclonal Ab against the part of the variable region (CDR1 mostly) of human monoclonal Ab (IgG).
In my previous trials I've injected mAb into the rabbit and cleaned the pAb on G-sepharose column. Eventually I’ve got the non specific pAb population i.e. it recognizes many common IgG determinants.
I have very little quantity of the mAb so I cannot slice it in order to rid of the common parts (like Fc for example).
Please advice if there are ways to produce determinant specific anti-Ab antibodies (poly or mono).
Thanks in advance.
Pery
I want to produce polyclonal Ab against the part of the variable region (CDR1 mostly) of human monoclonal Ab (IgG).
In my previous trials I've injected mAb into the rabbit and cleaned the pAb on G-sepharose column. Eventually I’ve got the non specific pAb population i.e. it recognizes many common IgG determinants.
I have very little quantity of the mAb so I cannot slice it in order to rid of the common parts (like Fc for example).
Please advice if there are ways to produce determinant specific anti-Ab antibodies (poly or mono).
Thanks in advance.
Pery
Post Comment
Related Communities
Recent Videos
Related Expert Forums
Recent Activity
Most Popular Trackers
RSS
Expert Activity
Community Members
