People frequently come onto this forum asking if they have anxiety. This puzzles me, as a long-time anxiety sufferer, because when you suffer from anxiety you know it. Remember, this is primarily an emotional condition usually with no known cause when it's chronic and with no known biological cause. If the only thing you're experiencing is something physiological, such as twitches, but you're not frightened irrationally to the point of panic or obsessiveness, then it's not anxiety. It can be stress -- everyone feels stress, including people with anxiety, it's a part of life, sometimes it gets difficult or seems difficult and that's stressful -- but if you're not feeling anxious, it's very unlikely anxiety is the problem. If you are feeling anxious, then you have anxiety. As the first answer tells you, neither you nor any of us can diagnose what's bothering you other than to offer possibilities -- for example, a lack of Vitamin D or magnesium can cause these symptoms, but we have no idea whether you suffer from this or other possible reasons or not. You most probably tweaked something. But if you find yourself obsessing over such things then you very well might in that case be developing an anxiety problem, because that's how anxious people operate. Before we suffered chronic anxiety, we either ignored these things or did something about them, but we didn't sit and stew endlessly over them.
Self diagnosis is highly likely to be incorrect since you have no medical training. If concerned about your health, see your doctor for a diagnosis. Many people have an itch here, a twitch there and an ouch that can happen at any time and there is nothing wrong with them, so likely you are ok too.