The best time to use the test is around noon or so, but anytime during the day or afternoon is fine. The only thing they usually recommend against is using first morning urine
is not recommended. The reason for this is that most women experience a surge in the morning, but it can take 4 hours for it to show up in your urine. Make sure to test at about the same time every day. After you get a positive you will ovulate 12-24 hours later so you should have intercourse within that time.