different test readings got me scared?

[yara_2010]

Dear Doctors

I'm the same one who posted I got confused with my blood 2 months ago and yesterday I was browsing in the internet and I went to the official cdc site which believe it is reliable site then I was reading about false positive results and how should be treated which is in this link http://www.cdc.gov/hiv/topics/testing/resources/guidelines/correctional-settings/section4.htm

My English is not that good or maybe I didn't understand what did mean so I got really scared when I have noticed that person who got initial false positive should re test again in 30 days using W.B. test method so:

1-I went to do 2 another tests yesterday  one was rapid and one was combo ab/an which came negatives so do you  think the two antibodies tests can replace  W.B. as  I did one w.b. along with PCR long time ago after the initial false positive

2- Do I need get 2 W.B. once on the day of the false positive and another one 30 days later and why 30 days to confirm my result?

4-Maybe I'm infected and  my body developed antibodies to the hiv virus during seroconversion period on that day 4 months ago their for got a different readings in test results like positive then negative then negative and might be positive again in the future ?

5-does this different readings depends on the window period or maybe from which part of the body the blood been taken ( right hand or left hand) ?

6- Is yesterday test enough which was four months and half after the initial positive considered conclusive or I should test again after 6 monhts as I got poitive 4 months ago?

[yara_2010]

Thanks alot Doctor, I appreciate your support and  your great work.  Have a great day Sir.

[Edward W Hook, MD]

1.  This make no difference.  No, you do not have HIV.
2.  Yes, these results are conclusive with respectr to your earlier false positve.

You have asked me to confirm what I said before. I did that.  I will not repeat myself agian.  Your false positve blood test did not inidcate that you have HIV.

EWH

[yara_2010]

Dear Sir

Thanks for your answers,

1-about the W.B. and PCR the two tests been done together and exactly 29 days after the initial false positive test which I got and this was the only W.B. test been performed   so does this will make any difference on your advise like doing another w.b. test?

2-without  telling my history if I had any exposure before the date the false positive do you think yesterday negative tests result ( Rapid and HIV Combo test) which is four months after the false positive is conclusive?

Thanks once again for your advise

[Edward W Hook, MD]

Welcome back to our Forum.  I have read your earlier interchange with Dr. Handsfield and can see that even though he reassured you that your initial test was falsely positive, it has continued to trouble you.  I hope that we can provide you with additional information to help resolve your concerns with this interaction.

Having a false positive test result can be very concerning and when you see different things said at different sites, that can only make a person worry even more.  Please realize that the advice provided on the CDC site you visited did not encourage PCR testing, just as we do not. The reason for this is that there is still not enough good information to know how to best use PCR test results and because PCR test results give more false positive test results than tests for antibodies (False negative tests on the other hand are very, very rare).  In your case however, following your initial false positive rapid test you have had additional negative antibody tests and a negative PCR test. These conclusively prove that you did not have HIV at the time of your initial blood test, despite its false positive result.  You need to believe this.  

Let me address your specific questions:

1.  Yes the combination of tests you have had conclusively prove that you do not have HIV.  You do not need to test further and do not need a Western blot.

2.   No you do not need a WB. The reason that the web site you visit suggests repeat WB is because if the initial test result had been caused by detection of antibodies early in the process of antibody development, before the entire process had finished, a repeat test would later show additional antibody formation.  At the time of repeat testing however, it would also be expected that a repeat antibody or ELISA test would be positive.  In your case, the repeat negative testing after the false positive and the negative PCR test more than take the place of the repeat WB.  It is not needed.

3.  At this time there is no evidence that you are infected and strong evidence that you do not. You need to move on  from this.

4.  Which hand the blood is taken from makes no difference. You are not in the window period.

5.  These results are conclusive.  You do not have HIV.  Take care.  EWH