Elisa 4th Generation - please help!

Hello, I wanna know what is the accuracy of 4th generation Elisa test in 4 weeks after possible hiv contact. Thank you guys!

[Frank303]

ELISA tests are licenced for testing after 90 days.

[mike_no]

Daniel,

If you fared a negative on a IV generation ELISA at 4th week, I would say that your chances of contracting HIV is extremely unlikely.

Now critics would speculate "WHY?"

Let me elaborate,

IV Gen ELISA is a DUO TEST (Antibody and Antigen Sandwich Test)

1. It actually looks for the P24 (protein) Antigen and Antibodies simultaneously.

2. A p24 Ag is almost always present in the body after acquiring the virus till the 4th week after infection and then it disappears

3. Once the p24 disappears, detectable amount of antibodies would be present.

4. Since the duo test detects for both p24 and AB simultaneously, either p24AG or AB is detected when tested after 3 weeks from acquiring the virus.

5. The duo test is at it's best at the 28th day.

6. An individual with a negative duo test at the 28th day and a 6 week negative can be absolutely certain that he's in the clear but following the book won't be a bad idea, you might still want to go ahead and get the 12 weeks test to completely eliminate the HIV from the scene.

Yes, IV Gen ELISA or equivalent can really minimize the testing window to 6 weeks
Tried Tested and Proven - Australia's SHCs probably are the world's best STD clinic system. The duo test is the routine HIV test and that's why they go with a shorter window of 6 weeks unlike in the other part of the world.

Daniel, you might want to start practicing your "whoo-hoo's"

[stelutu]

Mike, i really enjoyed your comment cause it's the kind of test I took.
BUT: on talking about combo tests, the one I took and also the one he asks us about seems to be the most stupid one. LOOK: it's the only one almost that did not detect a serious amount of P24:

Elisa Vironostika HIV Ag/Ab not good afer all:

http://www.aidsonline.com/pt/re/aids/fulltext.00002030-200201040-00018.htm;jsessionid=
HNCThvvdyXDQyD0zlYFb9lBp8nXvzJSfpfrlGJzZvv20zQpVwpkv!1253064403!181195628!8091!-1

As a resume: the presence of the p24 antigen was not detected, although it was high in the blood sample and the test sustained to pick up a 30ul/mg ! That's why Vironostika HIV seems to be less sensitive than all others combo !!!

I would really appreciate an answer, thanks!

[Teak]

This person really needs to move on.

[Teak]

Mike, there is a gap when the presents of antigen and detectable antibodies will not be detected in the fourth generation tests. The fourth generation test taken too early are not as reliable at the 3 generation tests.

[stelutu]

why do you have a problem with me being the fact i said THE TRUTH because you told mike the same thing as I. I took this type of test and that's why I looked into it and found out is useless as a duo, as DOCUMENTS based on studies state it is.

[Teak]

It's when you took the test.

[stelutu]

   Yes, I know,Teak. 15weeks post last high risk incident means conclusive.
   Believe me ,I wish to come back here in a month and say you are absolutely right and my body is not depressed enough not to produce Ab. I was diagnostically told to have Oral (and vaginal) Candida, witch I still have, that's why i find the test hard to believe.
    Today i saw a report showing my town of ~2mil people has >500 HIV/SIDA infected people. kinda freaky , you know....(

[Teak]

It looks as though you are not going to leave the forum on your own so I believe Medhelp help is going to have to put you on your way or move you do another forum. You don't have HIV and this is and HIV forum.

[mike_no]

I understand that there is a possibility of a brief second window period, during which p24 falls and HIV-antibody remains at low, undetectable level however, that's very theoretical in case that's taken at the 6th week. (4th week is slightly possible)

We would have to understand that the sensitivity of a Fourth generation ICMA test is >99.5% and specificity >99% to both B and non-B subtypes.

Hence, one could be rest assured that if he/ she has taken the test at the 6 week post exposure, the test result is highly unlikely to change ahead.However, a 12 week negative is mandatory for confirmatory reasons

[histress]

Hi,

You seem to have lot of Info about 4th Gen AB+AG test. I have tested negative at 3 week& 2 days (24th Day) and again only AB test on 32 day and also got negative AG+AB test on 52nd Day.

Can I take this as very safe (conclusive) that I'm fine.

[Vance2335]

Do not post on other people's threads.
You have been told you did not have a risk. So testing in your case is meaningless.