HIV-2 in Western Blot

[roltu]

Below is my lab report of Western Blot after 2.8 years of possible exposure. My question is regarding HIV-2. Can anyone please tell me Whether Western Blot looks for both HIV 1 and HIV 2 in same test. In this report it is said HIV 2 Negative, is that mean it has also checked for HIV -2 or a separate HIV -2 western blot is there?


Test Descriptions Observed Value Biological Reference

Interval

Infections :-

HIV I-gp 160 [ENV] NEGATIVE Negative

HIV I-gp 120 [ENV] NEGATIVE Negative

HIV I-P 66 [POL] NEGATIVE Negative

HIV I-P 55 [GAG] NEGATIVE Negative

HIV I-P 51 [POL] NEGATIVE Negative

HIV I-gp 41[ENV] NEGATIVE Negative

HIV I-P 39 [GAG] NEGATIVE Negative

HIV I-P 31 [POL] NEGATIVE Negative

HIV I - p 24 [GAG] NEGATIVE Negative

HIV I-P 17 [GAG] NEGATIVE Negative

HIV - II NEGATIVE Negative

Conclusion HIV I & II NEGATIVE

. .

. .

. .

. .

. .

Methodology : Western Blot - Auto Blot System 20

Remarks :

Western Blot for HIV I is a confirmatory test & not recommended for screening.

Criteria for HIV I Positive : 2 ENV(either of 2 ENV;gp160,gp41,gp120) and GAG ( p17, p 39, p24, p55 ) or POL

(p31,p51,p66).

Indeterminate for HIV I : Viral specific bands present,but pattern does not meet the criteria for HIV I Positive.

Repeat Testing(within 1 to 6 months) is must for any indeterminate result for HIV I

Indicative for HIV II result must be confirmed by HIV II specific western blot.

End of Report

[Teak]

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[roltu]

Teak I know u are very knowledgeable abt HIV. but i have read in India 2 Hiv-2 cases were not detected by Antibody test but by pcr. So due to my consistent symptoms I have given to test Hiv-2 Rna pcr. am awaiting for the report. I am very very much worried abt that. please help.

[roltu]

Ok thanks.

[Teak]

You don't have HIV.

[roltu]

Ok so is my anti HIV 1/2 negative confirms that I don't have HIV 2?

[Teak]

WB is not used to diagnose, it's used to confirm.

[roltu]

2.8 yrs last test 4 daysago.

[Vance2335]

How long ago was the risk and when was your last antibody test?

[roltu]

unprotected sex with CSW. Please I just want to know the WB was performed for both HIV 1 and 2 or not?

[Vance2335]

You had no need to test using WB.

What was your risk to begin with?

[roltu]

No, I tested for western Blot just to be sure of Hiv 2 as one study in India was that 2 people or negative for antibody though they had HIV 2. So please let me know whether I western blot I have done also tested for HIV 2 also. Is western blot test bot HIv 1 and 2 that is what I wanted to know.

[Vance2335]

So you never had a positive antibody test?

[roltu]

Hi vance,

                  Thanks for your reply. Ya I have taken several Hiv ab 1/2 , all negative. I am specially concerned abt HIV 2. So I am asking is the western blot I posted look for both Hiv 1 and 2?

[Vance2335]

WB is not the correct 1st test to take, an antibody test is. WB is a confirmation test.

What was your risk? Did you ever take an antibody test?

And yes it does