HIV Coinfection & RT-PCR detection

Hi Doctors,

I had two high risk sexual exposure  (Mar 5 and Mar11) , and I had a lot of ARS symptoms like sore throat,night sweet,swelling gland,dizzy and lost 20 pounds in a week after my second exposure . I was very scare within those three month. I did the RT-PCR test in the end of May(around two months after my last exposure) and the ELISA test on June 17 (which is almost 90 days after my last exposure). Both of the test results are negative.But it is getting more and more AIDS-ilke symptoms on my body including muscle and joint pain, felling numb at fingers and toes,night sweating, continuous losing weight,Gingivitis,losing hairs and come up with bunch of Purpura on my arms and knees. I read something on the internet about HIV CRFs( Circulating Recombinant Form) and  URFs       (Uniqure Recombinant Form) which could happen on co-infection or super-infection, So I wonder if that could happen on me since my two high risk sexual exposures are very close. And I was very unlucky to get infected twice. Is that possible the two different HIV strains recombined in my body to a new HIV strain which can not be detected by both ELISA and PCR?  thanks for Doctor's answer in advance.

[RainLover71]

Your negative elisa is proof that your negative and don,t have hiv.Your getting paranoid about the whole thing.Your safe.

[Teak]

Neither the Abbott RealTime nor the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 assay is intended to be used as a donor screening test for HIV-1 or as a diagnostic test to confirm the presence of HIV-1 infection.
Richard Klein
HIV/AIDS Program Director
Office of Special Health Issues
Food and Drug Administration

[hsiang00]

My negative test result was from COBAS TaqMan HIV-1 assay . Does that test mean anything for the detecting HIV-1 infection? Or it is just not having enough sample for screening the whole HIV strain coverage ? How about ELISA test? is that possible to have false negative result?What do you suggest me to do if I am still feeling wrong with my body even both tests result are negative?  Thanks for your time and answer.

[hsiang00]

thanks for your encourage and reply...I am crossing my finger and really hope I am fine.

[ricky_id]

By medical guidelines any approved test such as Elisa yields a conclusive result, if negative, at the 3rd month post-exposure or beyond. False positive may occur by then but not false negative.

[hsiang00]

Thanks for your reply. I was wondering if ELISA can also detect  a recombination HIV virus or mutation one.I heard that ELISA is used for detecting the antibody of HIV.Does Seroconversion happen on all the cases even though the virus has mutated? Sorry...I am really feeling well with my body.

[ricky_id]

Your conclusive negative is conclusive, don't overthink about this. Your diseases have nothing to do with HIV. I suggest you consult a doctor for those.