I took a test in my first trimester for hiv an it came bk negative why do I gotta take a hiv western reflex screen for do anybody know
hiv-1/hiv-2 ab w/reflex to dog confirmation. I got these results at the emergency room visit, due to chest pains. I took the test cause I had an encounter with a female of unknown status, 97 days prior.. My question is is this conclusive, I had a oral exam a week before witch was negative as well.. I need help, I'm scared..
yes, western blot counts. if 3 bands (parts of the hiv regions) show up in wb, then it's positive. else it's negative. elisa, ie oraquick, uses only 1 or 2 bands (for hiv-2) of synthetic antigen for antibody detection. it takes time for immune system to produce antibodies. pcr detects antigen directly (instead of antibodies) and must appear for antibodies to develop. if there's very little hiv, then very little antibodies are produced or there isn't enough time for antibodies to produce sufficient amounts, so elisa may be weak. 6+ months should be more than enough time for antibodies to show up (99.9+%)
The Elisa test is not on prepaidlab. This is why I am asking **if Western Blot counts**, I am aware that it is usually taken after the Elisa. I am also aware that it is only to confirm.
What I am saying is do my 2 Western Blot NEGATIVE tests (after 6+ months) really mean negative even though I didn't get the Elisa? If I had HIV I don't think the Western Blot would tell me negative, right?
Also known as: HIV Antibody, Preliminary Test/Confirmation (HIV-1/O/2). You took the wrong test! Preliminary means to proceed. Confirmation means to confirm!
http://www.prepaidlab.com/HIV-STD-Tests/HIV-SCREEN-Western-Blot-Confirmation-on-Positives-
Did you read this?
So you guys are telling me that 2 negative Western Blot's mean absolutely nothing without the Elisa?
How come if someone has a positive Elisa and a negative Western Blot then it's negative? That obviously means Western Blot is stronger than the Elisa.
Sorry if I'm coming out annoying but my question still isn't answered.
Testing guidelines require two positive test results and confirmed by a positive confirmative test such as the Western Blot.
An elisa test is used to screen for hiv by detecting antibodies produced by the immune system as a defence against the virus,if positive a western blot is then performed to detect antibodies against specific antigens.It,s a more sensitive test.
Btw, why do you need to get tested?
It would be better when there were two different tests to give you a final judgement, and ELISA would better come first, WB serves as a CONFIRMATIVE test.
So ELISA, and then WB when required (that is, when the ELISA test result comes out positive).
Please someone answer my question, I need to make a decision of what test to take by tomorrow! Or if I even need to at all!?
Are 2 negative Western Blots good enough to tell me or do I really need an Elisa??
Basically this is my question:
Can I take 2 Western Blot HIV tests and rely on them?
The site where I get my HIV testing from doesn't have the Elisa test as an option. I know this test usually comes first.
Instead I took 2 Western Blot tests for HIV and they were negative. Because I didn't take the Elisa, can I rely on the Western Blot results?
I really need help...
What's the difference, if you can explain to me. If Elisa must come first, and it technically doesn't matter if it's negative or positive because I'll end up having to take the western blot anyway?
My western blot was negative, that should mean I was negative right? I wouldn't need an Elisa because the western blot is confirmative and it's used as a "final" say anyway?
PCR isn't appropriate for testing HIV, but ELISA is.
If the ELISA test is done 3 months post-exposure and the result comes out negative, then you're conclusively negative. But if positive, you'll be referred to Western Blot as a CONFIRMATIVE test. If the WB result comes out negative but the previous ELISA positive, you conclusively don't have HIV.
So the appropriate one is ELISA first and IF required (read above) then followed up with Western Blot, but not other way around.