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MedHelp Member's Question

Western Blot vs. Elisa

by ConvincedOne, Oct 13, 2007 08:40AM

Tags: elisa, negative, talking

Is one more sensitive than the other. For example can i have a negative Elisa but positive Wester Blot? Does one test show more detailed information than the other?
Thank you

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Member Comments (2)

by ConvincedOne, Oct 13, 2007 08:42AM

Here is a pic of wester blot http://www.msichicago.org/ed/AIDS/img/hivtst5.GIF
So is it possible the the western blot might be more sensitive?

by pep01, Oct 13, 2007 09:53AM

yes

by ConvincedOne, Oct 13, 2007 11:44AM

To: pep01

Please don't answer if you don't have clue what you're talking about. From what i found Western blot is less sensitive but more specific, that is, one that has a lower rate of producing a false-positive result.

by ImStupid, Oct 13, 2007 11:56AM

To: ConvincedOne

I believe your indication to be correct. Western Blot is used to back up a positve Elisa. With out testing positive on a western blot, you are not considered "positive". By no means an expert...but thats what I've picked up through the numerous threads I've read.

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