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please help me becuase i dont know read that much in english....they send it to me by Mail from the clinc anyway plz help me out and explin to me this result.................this is my test result................................HIV VIRAL LOAD BY TAQMAN
HIV VIRAL LOAD <20 copies/mL
*****INTERPRETATION OF HIV-1 VIRAL LOAD RESULT:
HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 WAS NOT DETECTED IN THE GIVEN SPECIMEN.
HIV VIRAL LOAD BY TAQMANHIV
VIRAL LOAD BY TAQMAN
• The viral load provides an accurate estimate of the level of HIV replication.
• Quantitation of HIV Viral load is important as it serves to be a prognostic marker of HIV infection.
• It is used for establishing baseline levels in patients before initiation of the therapy and for monitoring the disease progression.
• A sudden rise in viral load values may indicate emergence of resistant strains during therapy.
Cobas Ampliprep/Cobas Taqman
• HIV viral load is expressed as copies/ml.
• For conversion to WHO International Units (IU): 1 IU corresponds to approximately 0.6 copies/mL.
• The lower limit of detection of this test is 20 copies/ml
• Viral load below 10,000 copies/mL is generally considered "low".
• An increase or decrease in viral load of more than three-fold may be considered significant.
• Viral load may vary considerably. Laboratory variations, acute illness or recent vaccination can lead to elevations or variability in viral loads. However variations of less
than 0.5 log copies/ml should not be considered as significant.
• Values below 20 copies/ml do not exclude possibility of an infection. It may indicate viral load below detection limit of the assay.
• Follow up viral load below the detection limit of the assay may indicate resolution of the infection after the therapy. Reappearance or increasing viral load may indicate
relapse of resistance to the therapy.
• Viral load values are known to be affected due to other clinical conditions such as acute illness or recent vaccination. Hence viral load should be interpreted in conjunction
with clinical history, clinical status, and other diagnostic parameters.
1.Viral Load is a monitoring test and hence should not be used for screening or diagnostic purpose.
2.Wide variations in viral load have been observed due to following reasons:
a) Use of different technologies/platforms for follow-up testing: It is recommended to monitor patients using same technology
b) Non-adherence to specimen collection protocol. Hence it is recommended immediately to freeze the EDTA Plasma after collection and separation.
• PCR is a highly sensitive technique; common reasons for paradoxical results are contamination during specimen collection, selection of inappropriate specimens and
inappropriate...........................................plzzzzzzzzzzzzz help is it Negative and Conclusive؟؟؟؟؟؟
Neither the Abbott RealTime nor the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 assay is intended to be used as a donor screening test for HIV-1 or as a diagnostic test to confirm the presence of HIV-1 infection.
HIV/AIDS Program Director
Office of Special Health Issues
Food and Drug Administration
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