Calcium increased with 1.25ohd3 and not by tested level of vitd25oh

[stef2011]

http://www.vitamindwiki.com/Calcium+intestinal+absorption+increased+with+active%2C+but+not+tested+level+of+vitamin+D+%E2%80%93+May+2014

25-Hydroxyvitamin D level does not reflect intestinal calcium absorption: an assay using strontium as a surrogate marker

j Bone Miner Metab. 2014 May 24. [Epub ahead of print]
Camargo MB1, Vilaça T, Hayashi LF, Rocha OG, Lazaretti-Castro M.
1Division of Endocrinology, School of Medicine, Federal University of São Paulo/UNIFESP, Avenida Nove de Julho, 5581, Apto. 30, Jardim Paulista, São Paulo, SP, CEP 01407-200, Brazil, ***@****.

There is conflicting evidence as to the optimal serum 25-hydroxyvitamin D [25(OH)D] concentration for intestinal calcium absorption (Abs-Ca). Our purpose was to assess the relationship between vitamin D status and Abs-Ca in postmenopausal women. Fifty volunteers with low bone mass were grouped according to their serum 25(OH)D concentration as follows: mild deficient, <50 nmol/L (DEF) and sufficient, ≥75 nmol/L (SUF). The subjects were submitted to an oral strontium overload test to assess their Abs-Ca. Fasting blood samples were obtained to perform the relevant hormonal and biochemical tests. After the subjects received the test solution, blood samples were drawn at 30, 60, 120, and 240 min to determine the strontium concentrations. Abs-Ca was indirectly expressed as the area under the serum strontium concentration curve (AUC). A repeated measures ANOVA was performed to determine the differences among the groups. Pearson's correlation and multiple linear regression analysis were used to study the associations between the variables. The mean 25(OH)D and 1,25-dihydroxyvitamin D 1,25(OH)2D concentrations differed between the groups (SUF vs. DEF) as follows: 98.7 ± 18.2 vs. 38.4 ± 8.5 nmol/L (p < 0.001) and 36.2 ± 10.2 vs. 24.9 ± 4.6 pg/mL (p < 0.001), respectively. There was no statistically significant difference between the groups for parathyroid hormone and AUC.
Only 1,25(OH)2D influenced the strontium absorption in the last 2 h of the test. In the studied population, no correlation between levels of 25(OH)D and Abs-Ca was found. Only 1,25(OH)2D influenced Abs-Ca as measured by a strontium absorption test.

[stef2011]

subtitles of the interview are now in english too, the protocol is about using high dose vitamin d to regulate immune system in many diseases.MS is now confirmed to be epstein bar virus active infection.they talk about many other diseases.
the italian who did the interview cured his disease (not MS) too applying this protocol

https://www.youtube.com/watch?v=hOfO29rL-gI

link to single questions since the interview is very long

https://translate.google.com/translate?sl=it&tl=en&js=y&prev=_t&hl=it&ie=UTF-8&u=http%3A%2F%2Fwww.leonardorubini.org%2F2014%2F04%2Fil-re-e-nudo.html&edit-text=

[stef2011]

to define your trial:
pth must go lower range 11-20 to confirm that vit d was having any biological activity.if PTH remained more than that vit d was doing nothing in your system

[Rome70]

I was testing 1.25 oh and it is positively correlated with 25oh

Date/25(oh)/hbsag/dna/vit d3/1,25 oh
July/58/1590/<20/10000
Aug/64/1426/<20/10000
Sep/70/1891/86/10000
Nov/34/1856/56/5000/82
Dec/48/1907/50/10000/89
Feb/22/1530/<20/4000/75

Definately negative correlation vit D and hbsAg ....

[stef2011]

so to keep in check all parameters involved one can check:
vitd25oh
vitd 1.25oh
pth
nagalase
urine calcium, ionized and serum calcium

the experience of me and my sister at vitd25oh 150ng/ml is:
vitd1.25oh not checked yet but probably normal because calcium is low

pth from medium to high, means calcium is low.when calcium absorption gets high pth goes down

nagalase high, this is produced by viruses and blocks vit d macrophages activation

urine calcium, ionized and serum calcium, all low and declined from baseline before vit d supplementation

[stef2011]

this also explains why we are seeing lower calcium levels by increasing vitd25oh

hypotesis:
1.25oh is increased on chronic diseases because macrophages dont get activated.this is due to high nagalase produced by viruses and low vitd25oh which prevents vdr activation and so macrophages activation

when 25oh d3 levels increase there is more macrophages activation and so 1.25oh goes down and this is why we see both calcium and 1,25oh going down as vitd25oh goes up

latest studies found vitd25oh action on genes the same as 1.25oh, both types probably exist because action on genes by vit d must be stable but vitd25oh cannot be stable because sun irradiation is variable.my hypotesis is that 1.25oh is just a back-up of 25oh so that we have stable levels