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Regarding HBV DNA quantitative test results
hi,
i am a male and was found positive for Hepatitis B about two years back. doctors say i am just a carrier. My recent LFT gave SGPT count as 56 which is out of the range of 13-40. so i got my HBV DNA quantitative test done. the result came out to be as "under detectable limit". what does this conclude?
i don't consume alcohol and am a healthy person as a whole.

1) Is the hepatitis B virus not replicating in my body?
2) Also i want to know the chances that my wife who has taken the vaccination for Hepatitis B gets this virus from me?
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Hi Stef;

kindly find below results

HBsAg(CMIA)  250 IU/ml

SGPT(ALT)   60 IU/ml

SGOT(AST)  45 IU/ml

HBV DNA, QUANTITATIVE REAL TIME PCR ( Real Time PCR using
Cobas Ampliprep & TaqMan)   658 IU/ml

AIDS(HIV-I, HIV-II) ANTIBODIES FOR HIV (I&II)/P24 ANTIGEN 0.10

PT/PT-INR
Prothrombin Time
Test 12.5 second(unit)
control 11.0 second(unit)
INR 1.1

Anti HBe Method by chemilumiscence Result Non - Reactive(sample/cut-off ratio(s/co)-1.24)

HBeAg  Method by chemilumiscence Result Non - Reactive(sample/cut-off ratio(s/co)-0.3)

HBsAg Quantification 3626 IU/ml


Fibro Scan
Cap(db/m)                              E(KPa)
Median      370                       Median            6.5
IQR*           25                        IQR*               1.2
IQR*/med    7%                       IQR*/med        18%

plz help and suggets me any treatment to make HBsAG negative
Waiting your usual Advise



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Hi Stef;

kindly find below results

HBsAg(CMIA)  250 IU/ml

SGPT(ALT)   60 IU/ml

SGOT(AST)  45 IU/ml

HBV DNA, QUANTITATIVE REAL TIME PCR ( Real Time PCR using
Cobas Ampliprep & TaqMan)   658 IU/ml

AIDS(HIV-I, HIV-II) ANTIBODIES FOR HIV (I&II)/P24 ANTIGEN 0.10

PT/PT-INR
Prothrombin Time
Test 12.5 second(unit)
control 11.0 second(unit)
INR 1.1

Anti HBe Method by chemilumiscence Result Non - Reactive(sample/cut-off ratio(s/co)-1.24)

HBeAg  Method by chemilumiscence Result Non - Reactive(sample/cut-off ratio(s/co)-0.3)

HBsAg Quantification 3626 IU/ml

Fibro Scan
Cap(db/m)                              E(KPa)
Median      370                       Median            6.5
IQR*           25                        IQR*               1.2
IQR*/med    7%                       IQR*/med        18%

plz help and suggets me any treatment to make HBsAG negative
Waiting your usual Advise
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iam hepatities b patient.in resent result i got hbv  dna viral load is  717iu/ml.is there any serious please help me..
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hbvdna is useless to know how you are, you must check fibroscan to know if you have liver damage or any damage from hbv
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Hi Stef;

kindly find below results

HBsAg(CMIA)  250 IU/ml

SGPT(ALT)   60 IU/ml

SGOT(AST)  45 IU/ml

HBV DNA, QUANTITATIVE REAL TIME PCR ( Real Time PCR using
Cobas Ampliprep & TaqMan)   658 IU/ml

AIDS(HIV-I, HIV-II) ANTIBODIES FOR HIV (I&II)/P24 ANTIGEN 0.10

PT/PT-INR
Prothrombin Time
Test 12.5 second(unit)
control 11.0 second(unit)
INR 1.1

Anti HBe Method by chemilumiscence Result Non - Reactive(sample/cut-off ratio(s/co)-1.24)

HBeAg  Method by chemilumiscence Result Non - Reactive(sample/cut-off ratio(s/co)-0.3)

HBsAg Quantification 3626 IU/ml

Fibro Scan
Cap(db/m)                              E(KPa)
Median      370                       Median            6.5
IQR*           25                        IQR*               1.2
IQR*/med    7%                       IQR*/med        18%

plz help and suggets me any treatment to make HBsAG negative
Waiting your usual Advise
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Hi, I have recovered from Hep B for 11 years. I have test done january showing negative Hbsag, postive anti HBS ,SGPT 47.and non detectable HBVDNA, the doctor suggested to have another test done this august.. now came the result, still HbsAg negative and anti Hbs Positive but my SGPT is 55, what can u say about this?Is this just okay?
Thank you.
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I tested my blood and I saw HBV DNA Quantative by (TaqMan) > 110000000, so have any drug can cure it?



wait for your reply.

Thanks.
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HBV DNA Quantative by (TaqMan) > 110000000

you are probably healthy with that level, the higher the virus the less damage usually because there is no immune response

remember it is not the virus to make the damage but your immune system, so you need to check if you are an healthy immune tollerant carrier, immune tollerant have:
hbvdna in the millions
hbsag 20-100.000iu/ml
ast/alt normal
liver fibroscan less than 6kpa

no drugs needed if so, you are healthy
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you have abnormal alt, normal is 19 or less

you need to check the reason which can be:
occult hbv, very sensitive hbvdna can confirm this

fatty liver

bad quality food with chemicals, try to eat organic fresh food only without any chemicals
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yes, i have a fatty liver. so i guess i need to check what i eat.just like that.
Thanks.
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Im sure this is not an occult HBV,bec I have zero HBVDNA count and Negative HBsAG ( 0.35), Positive,Anti Hbs ( 233.95).
Fatty liver might be the cause of my abnormal SGPT, but its not quite high, here, normal range from 5-31.I have fatty liver, Yes.
Thanks.
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I am 37 yrs old and HbsAg positive with HBV DNA showing 4540642IU/ml = 26426536 copies/ml, HbeAg Negative and ANTI HbeAg Positive, ALT is 66 and AST is 48 Rest all liver parameters are withing normal range. Please advise. i am feeling mild pain under my right lower ribs. doctor is suggesting Interfero Inj and every week for 4 months and i cannot afford that due to its cost. The alternative he told to take Tenovir on each day for three months and then undergo the blood testsagain. please advice
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Hi, I’m M. Talib and I’m 27 years old now (date of birth is 15/10/1985). I belong to Karachi, Pakistan.
I am having hepatitis B virus in my body that is inactive. After 5 months I will get married. I’m worried about my wife (lolls, actually at the moment fiancée) that after marriage, whether my virus will transfer to my wife or not? What should I do now?  My all reports (with test dates and test results) are as follows: Kindly help me.

First time test from Agha Khan Hospital (dated on 01/03/2012) :
HBsAg (test performed by ECLIA) ---------Result= reactive (2267)
Hepatitis C antibody (test performed by ECLIA) ------ Result = non reactive 0.107

Second time test from Liaquat National Hospital (dated on 22/03/2012):
HBsAg (test performed by MEIA) ---------Result= reactive (262.54)
------------------------------------------
After this, I went to Liaquat National Hospital and there a doctor suggested me following further tests:

(I’m mentioning here with results)
Dated on 02/04/2012

1. HBV DNA PCR Quantitative--- = HBV DNA detected in sample, viral load 328 IU/ml

2. HBe Antigen-------------------------- = (performed by elisa) non-ractive 0.034
3. Anti HBe Antibody-------------= (performed by elisa) positive 0.348
4. Anti HDV-------------------------= (performed by elisa) negative 2.527
5. CBC:
 HAEMOGLOBIN = 15.1 g/dl
 RBC = 4.8 10E12/L
 HAEMATOCRIT (PVC) = 42 %
 MCV = 88ft
 MCH = 31 pg
 MCHC = 36 %
 TOTAL LEUCOCYTE COUNT = 4.3 ×10E9L
 DIFFERENTIAL LEUCOCYTE COUNT
NEUTROPHILS = 55 %
LYMPHOCYTES = 34 %
EOSINOPHILS = 0.6 %
 PLATELETS = 196 ×10E9/L
 MORPHOLOGY = NORMOCHROMIC. Normocytic
6. LFT:
 S. TOTAL BILIRUBIN=0.49
 S. Direct BILIRUBIN=0.16
 S. INDIRECT BILIRUBIN=0.33
 SGPT (ALT) = 38
 ALKALINE PHOSPHATATE = 102
 GAMMA GT = 18
 SGOT (AST) = 27
7. PI----------------------- = 14.5 seconds
8. Albumin--------------------------= 4.56
9. U/S upper abdomen-------------- = It was found ok
10. Creatinine------------------------ = 0.73
11. BGR (Blood Glucose Random) ------- = 122
12. SERUM TSH------------------------ = 1.11

 I showed the above twelve reports to the doctor. And he told me that no need to worry because the virus (hepatitis B) in my body is inactive. But I need two tests after every 5-6 months for observation:
ALT & HBsAg

 The doctor also suggested me to use folic acid (5mg) for one month only. He also told me that in the presence of virus (although it is inactive) I can not do vaccination.

Now After passing 6 months almost, I again tested myself from hospital and my reports are as follows (Dated on 27/09/2012):
 SGPT (ALT) = 36

 HBsAg (test performed by ECLIA)= Reactive 2670
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hayee...I m tens....
I hv Hep B Positive
ALT 39 out of 43
AST 33
HBV DNA 3.5*10^4 (35000 copies/ml)
Ultrasound Report Normal..

Is it Choronic....? am i need treatment at this stage...? help me  
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Hi steff...My ALT/AST Normal and Ultrasound report also normal...my HBV DNA show viral load 35000 copies/ml (6013 IU/ml)....please suggets me ..is there any need of treatment or not....? and is it acute or chornic ?
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i m 25 year old....
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Dear Stef,

HBsAg = 2699
HBV DNA = 133800 IU/ml
HBe Antigen = reactive (228.6) performed by ECLIA
HBe Antibody= non-reactive(1.85) performed by ECLIA

ALT = 61

i am 29 years old and living in Pak. kindly suggest me if any treatment require or not. need your help. as i am unable to join my new job.
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pakistan doesn t have hbsag quant

this is not hbsag quant and the number has no unit (hbsag=2699 and not 2699iu/ml), this is a useless test if possible ask for money back
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Thanks stef,

but what will i have to do further. what about other tests conducted. i.e HBV DNA quantitative, HBe Antigen, HBe Antibody etc. your value able suggestion needed. either treatment required or not ?
I consult physcian locally in Pak and they recommend me to start treatment.
1- etv
2-tdf
they said to use above two pills once a day and come after one month with ALT.
what u suggest?

waiting for your reply.
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hi stef
           iam from india. iam hbv+. by the first hbv dna test igot result as
  on jan2012  hepatitis b virus dna quantative pcr 29752971 IU/mL
  
doctor suggest to use (entaliv0.5mg) tablets
  on oct 2012 hepatitis b virus dna quantative pcr 1490 IU/mL

then i stopped using the tablets for 2-3 months. by consulting the doctor he advised to do the same test again
  on jun 2013 hepatitis b virus dna quantative pcr 115392 IU/mL

can i know wether my liver is damaged or not?
is there any chance to make this hbv negetive?
  to reduce the count should i follow any food diet?
if it is possible please guide me
   please reply me
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first of all find a liver specialist that knows hbv, that doctor knows nothing about hbv and hbv drugs.the antivirals dont clear hbv they just lower replication so if you want to take an antiviral you have to take it for life

can i know wether my liver is damaged or not?
yes you  need a fibroscan to know this

is there any chance to make this hbv negetive?

hbv negative is a useless test, if it gets negative it does not mean clearance of hbv.it is is test just to know if the antiviral is lowering hbv replication, it has nothing to do with clearance of infection.
to know if you are clearing hbv you need hbsag quantity, not hbvdna quantity

to reduce the count should i follow any food diet?

no, diets and vitamins have no effect on virus (except vit d3), this is useful only to keep the liver healthy and lessen the damage but it is not effective on the virus.follow posts about vitamin d, it looks like increasing vit d boosts immune system but it is not known yet if this lowers replication

there is only one therapy to clear infection in a very high percentage and it takes 4-5years minimum:
first you need to take tenofovir for about 3 years, this will rescue your immune system

then you need to add on to tenofovir peginterferon, this combo of the 2 drugs will clear hbv in 50% of cases by 1 year and will lower hbsag to inactive hbv on the rest of 40% by second year of combo, only 10% seems not to respond.studies to define better this 40% which gains low hbsag are underway
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My HBsAg result is >250 IU/ml . I had got HBV immediately when i was born . I didn't get vaccinated . Need to whether its acute , chronic or carrier?
Is it curable ?
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Since you were infected at birth, then you are chronic, if of course you are older than 6 months...
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Dear Stef,
                  I am HbsAg +ve donno since when. I got to know when i donated blood in 2011 december. Tested HBV DNA on Sep 2012 and it is 4540642IU/ml = 26426536 copies/ml, ALT - 66, AST - 48, rest all LFT is within normal range, i was feeling mild pain under the right lower ribs, HbeAg is Negative and Anti HbeAg is +ve.
Doc advise to take tdf for six months and test again.
Aug 2013 report is
HBV DNA is < 6 iu/ ml = < 35 copies/ml.
Alt 26 and Ast 26. Rest all reports are within normal.
What is the next course of treatmant? Pls advise. I am feeling occassional mild pain under the right lower ribs.
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kindly interpret results below;

HBsAG II quantification =37810.0 IU/ml
HBV Viral load >110,000,000 IU/ml
ANti HBC LgM (CMIA) = 0.16      C.O.V. 1.000 nonreactive
ANti HBS= 0.33 (rate note 0.33); cut off 10.0 ECLIA method
HBsAG=reactive (rate note 3241.11) cut off 2.0 ECLIA METHOD
Anti HBC lgG=reactive (rate note 12.19) cut off 1.0
SGPT = 22
Anti HCV = non reactiveAN\\
Anti HBe = reactive
Anti HBe Antigen = non reactive


the doctor has prescribed me to take  baraclude 0.05 mg and I don't know if I will start the medication. Pls. advise. Thanks
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tenofovir is the most potent drug on hbv, has no resistance and is cheaper than entecavir, why use entecavir?

in our community we think most doctors are instructed or just believe drug sellers lies about tenofovir having sides effects while entecavir having no sides effects.it is actually the opposite, long term use of entecavir has effects on mitocondria and human dna while tenofovir doesn t have.entecavir is also under monitoring for cancer because in human trials there was lung cancer on mices while no cancer on monkeys

so in the end tenofovir is better
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see liver condition, if ok then no need for anti viral. think twice before starting antiviral.
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i have a hepa b..this is my HBVDNA result..

5,652 IU/ML
32,895 COPIES / ML DETECTABLE RANGE W/ METHOD IS 6~ 110,000,000 IU/ML  (35-640,200,00 copies /ml)
what did they mean?

the doctor told me to no need for treatment,is it true?
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i'm wait for your reply..i hope u can advice me..thank you
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Please help me to interpret this result;

< 6 IU/ml < 35 copies/ml The method used for HBV DNA Viral Load PCR is COBAS TaqMan HBV Test. The detectable range with this method is 6 - 110,000,000 IU/ml (35 - 640,200,000 copies/ml). The quantification is homogenous for all HBV genotypes tested.


do this mean that I have a virus on my blood? please help coz I don't know what to do.

Thank you so much,
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hbvdna test alone is useless to say anything.the virus is present anyway indipendently from hbvdna results

you need hbsag quantity in iu/ml and fibroscan to make hbvdna test useful

also hbv genotype, hbeag, hbeab, ast-alt are needed to define status
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Sir I am a man of 47 years old ,
I didn’t do any hapatius b test before, my first test (days ago) shows that : HBs Ag + , sgot=24 , sgpt = 24
HBA Quantitative pcr shows 76 IU/Ml viral load.
do I need treatement or I have to wait maybe my body will get rid of the virus since the viral load is low , and what treatement is needed if any ?
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do I need treatement or I have to wait maybe my body will get rid of the virus since the viral load is low , and what treatement is needed if any ?

hbvdna viral load has nothing to do with the infection in the liver, clerance of the infection or hbv cure by any therapy.it is hbsag quantitative in iu/ml to show infected cells in the liver, prediction of cure by therapy and so on, so have hbsag quantitative test if you want to know, the test is very cheap and result very fast

check also hbv genotype and in case hbsag is less than 1000-1500iu/ml you have highest chances to clear hbv on peginterferon
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this is the quantity result

hbsag 1795.16 reactive
hbs non reactive
hbeag .34 non reactive
anti hbe .01 reactive anti hav .45 non reactive
hbc total 12.61 reactive hcv .13 non reactive

The doctor said no need for treatment...
i hope you can explain me clearly..

is there a possibility to remove this virus?

i will wait your response...thank you
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this is the quantity result

hbsag 1795.16 reactive
hbs non reactive
hbeag .34 non reactive
anti hbe .01 reactive anti hav .45 non reactive
hbc total 12.61 reactive hcv .13 non reactive

< 6 IU/ml < 35 copies/ml The method used for HBV DNA Viral Load PCR is COBAS TaqMan HBV Test. The detectable range with this method is 6 - 110,000,000 IU/ml (35 - 640,200,000 copies/ml). The quantification is homogenous for all HBV genotypes tested.


The doctor said no need for treatment...
i hope you can explain me clearly..

is there a possibility to remove this virus?

i will wait your response...thank you


wat is the best medicine to cure?
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hbsag 1795.16 reactive

this is not a quantity, this is a number without a unit, this is the fraud obsolete test sold as quantitative by labs making this fraud for years.the number is the reagent used nothing else, at least you can require a money back for such a useless test

only two machines widely available since 2000 can make hbsag quantity in iu/ml, abbott architect or roches elecsys, all other machines decades older than 2000 are obsolete and useless and cannot even detect all types of hbsag making a lot of false negatives

without the tests for hbsag quantification and hbv genotype how could you monitor treatment?

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imean hbsag 1795.16 iu/ml reactive..
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with iu/ml as i have mentioned..
i will wait your reply..tnx
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you can try tenofovir for 3 years and then peginterferon add on to clear hbv or peginterferon monotherapy for 24 weeks and if no hbsag decline go for the previous suggested sequential treatment
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Hi , stef
I,m a man of 47 with  HBs Ag positive

my LFT results are normal sgot=24 , sgpt = 24

HBA DNA pcr was76 IU/Ml in sept. 2013.
HBA DNA pcr became 44 IU/Ml in Oct. 2013.

HbsAg quantity is 2075 IU/Ml now in 7th Nov. 2013

what treatment is needed to clear HbsAg and how long treatment will take
Thanks alot for your help
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just finished my 48 wks of peginterferon and my hbsag quantification result is 143 and hvbdna is <6. We solicited dr advise if we can continue the treatment to 60 or 72 weeks, ans is to totally stop. Right now no medication, just monitoring.

Is anyone there can confirm if my doc advise is just right for my case.
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It would be best to start tenofovir first, you are Lmost 50yo and there s increased hcc and cirrhosis risk, then follow hbsag when 1500iu/ml add pegintf

U must also keep your vitd levels above 90ng/ml to keep good immune responce and healthy liver
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Hi Steff,
May I need some medication based on my blood test as under

HBs Ag---2624.73
ALT --- 72
AST----39
Hepatitis Be Ab.............. Non Reactive
Hepatitis Delta antibody --- Non Reactive
HBV DNA Quantitative ..... Detected (512278 IU/ml)
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HBs Ag---2624.73

what is the unit?

fibroscan is also needed to define all situation but all guidelines suggest therapy because high hbvdna and abnormal alt (norm alt is 30 for men)
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Hi
I am 30yr old and hbsag >250 iu/ml
hbv qpcr undetectable.
please guide as i am very nervous.
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hi ritu...
same problem is with my husband..i am also very worried..can you please guide me .
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hi
my sis ,age 25 , is proposed for BMT ( hodgkin lymphoma relaps )
now she has been diagnosed as hbv reactvie following are her test result's :
HBsAg >250
HBeAntigen (cmia) 1467.73
SGPT 27
SGOT 19
fibroMeter F1
Infla meter A1/A2
hbv dna (quantitative) >100,000,000
doc. has prescribed entecavir(.5 mg + tenofovir (300mg)
and has delayed BMT for 1 month
please suggest that is it necessary to delay bmt coz her cancer is aggressive one..??
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my HBV VIRAL LOAD BY TAQ MAN > 106000. What is the actual reference range of this reasult ?
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hbvdna test alone is of very little meaning, you need hbsag quant in iu/ml, fibroscan, liver function, hbeag/hbeab, hbv gentoype to have an idea of the status
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my age 27+ . last dec 2014 found hepatitis B , now
SGOT/AST-55
SGPT/ALT-90
HbeAg- Reactive ( 330.859)
HBV DNA quantification-COBAS TaqMan- 10,01,983 IU/mL
Log Value 6.00

Please suggest my Liver Condition ?
What type of Precision required ?
Is possible to normal situation should be in future ?
this is primarily through blood exposure

Pls Help me

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