Aa
A
A
Close
Avatar universal
effect of nucs for 5-11years on intrahbvdna cccdna
http://www.professionalabstracts.com/ilc2014/planner/index.php?go=abstract&action=abstract_show&absno=2080&

THE EFFECT OF LONG-TERM NUCLEOSIDE/TIDE ANALOGUE TREATMENT ON INTRAHEPATIC HBV DNA AND COVALENTLY CLOSED CIRCULAR DNA IN CHRONIC HEPATITIS B PATIENTS Print

D.K. Wong1,2, C.-L. Lai1,2, P. Ip3, M. Kopaniszen1, W.-K. Seto1, J. Fung1,2, F.-Y. Huang1, R. Wu1, C. Cheng1, V. Ngai1, M.-F. Yuen1,2
1Medicine, 2State Key Laboratory for Liver Research, 3Pathology, University of Hong Kong, Hong Kong, Hong Kong
E-mail: ***@****

Background and aims: Nucleoside/tide analogue (NA) treatment suppresses serum HBV DNA to very low levels. However, their long-term effects on intrahepatic HBV DNA (ihHBV-DNA) and covalently closed circular DNA (cccDNA) have never been documented.
Methods: Patients on long-term continuous (5 - 11 years) NA (lamivudine/adefovir/telbivudine/entecavir/tenofovir) were recruited. All patients had 3 liver biopsies: at baseline, after 1 year of treatment and at the last follow-up. Serum HBV DNA was measured by the COBAS TaqMan HBV Test (Roche Diagnostics). ihHBV-DNA and cccDNA were assayed by real-time PCR as previously described.1
Results: To date, 10 patients had undergone liver biopsies at the last follow-up, all of whom had either 100mg lamivudine or 0.5mg entecavir at baseline and had been switched to 1.0mg entecavir during follow-up (median follow-up: 11.4 years; range 10.7 - 11.5 years). At baseline, the median serum HBV DNA, ihHBV-DNA and cccDNA levels were 6.66 logIU/mL, 273 copies/cell, and 3.2 copies/cell, respectively. At the time of the last biopsies, although all 10 patients had serum HBV DNA levels below detection limit (< 20 IU/mL; p< 0.0001 comparing to baseline), all had detectable ihHBV-DNA and cccDNA. However, the ihHBV-DNA and cccDNA levels were greatly reduced, with median values of 0.45 and 0.04 copies/cell, respectively, and median percentage reductions of 99.9% and 98.2%, respectively (both p< 0.0001; compared to baseline levels).
Conclusions: Long-term NA treatment significantly reduced ihHBV-DNA and cccDNA, though small amount of these were still present. Whether more prolonged therapy can eradicate them is still unknown.
1. Wong et al, (2013) Clin. Gastro. Hepatol. 11:1004-10


Assigned speakers:
Ching-Lung Lai , University of Hong Kong , Hong Kong , Hong Kong

Assigned in sessions:
12.04.2014, 09:00-18:00, Poster, 7C - VIRAL HEPATITIS: HEPATITIS B & D - CLINICAL (THERAPY, NEW COMPOUNDS, RESISTANCE), Poster Exhibition
Cancel
4 Answers
Page 1 of 1
Avatar universal
these reports are so poor, why not report serum hbsag?

too bad lam and etv only
Comment
Cancel
Comment
Avatar universal
Comment
Comment
Submit Comment
Avatar universal
doesn't cccdna quantity in cell gives better understanding of what is going on inside the liver rather then measuring hbsag in blood?
Comment
Cancel
Comment
Avatar universal
Comment
Comment
Submit Comment
Avatar universal
Though not a medical professional - looks very encouraging to me and a big relieve.
Comment
Cancel
Comment
Avatar universal
Comment
Comment
Submit Comment
Avatar universal
that s just to have an idea of the immune suppression from hbsag and balance with immune response, which you can t have from cccdna level.this will tell you if peg would clear in all of them
Comment
Cancel
Comment
Avatar universal
Comment
Comment
Submit Comment
Your Answer
Avatar universal
Answer
Know how to answer? Tap here to leave your answer...
Answer
Submit Answer
A
A
Blank
Weight Tracker
Weight Tracker
Start Tracking Now
Hepatitis B Community Resources
RSS Expert Activity
233488 tn?1310696703
Blank
Marathon Running Done Over Many Yea...
05/15 by John C Hagan III, MD, FACS, FAAOBlank
233488 tn?1310696703
Blank
New Article on Multifocal IOL vs &q...
05/15 by John C Hagan III, MD, FACS, FAAOBlank
748543 tn?1463449675
Blank
TMJ/TMJ The Connection Between Teet...
01/15 by Hamidreza Nassery , DMD, FICOI, FAGD, FICCMOBlank
Top Hepatitis Answerers