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hbsag

hi stefano wat is hbsag quantitative by elisa method
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Avatar_m_tn
the number is the reagent quantity not hbsag, elisa is a very old method born for hiv testing or even before, if the reagent is among a certain number the result is pos and if below neg, there is no quantification

it is very stupid that labs give the number which doesn t reflect anything, i think it is to fool the clients because in italy we have all tests and there is no stupid number but only positive or negative on this test
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Avatar_m_tn
ok ok. when i asked in a diagnostic centre they told this. they even doesnt know about abbott architect. i dont know how to find it, then is that possible by eclia method?
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Avatar_m_tn
no it is not

i have seen an old study made by brunetto where she explains how to get hbsag quantification by other machines but i don t think labs have good technicians, most of them don t even know what they do, they just read the numbers on the machines, so even if we explain a method they don t or are not able to do it

try search on google " brunetto hbsag quantification cov 1000" put search period between 2004 and 2008 and also try the image search
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Avatar_m_tn
i am sorry now i recalled the article it is about dilutions because abbott architect gets hbsag until 250iu/ml then you have to make manual diluitions to get higher numbers and of course cronic (chronic) carriers have all higher numbers in the 3-5logs range (250 is the 2 logs range)

i have seen there are other machines for hbsag quantifications but they are all new ones, the oldest one is abbott architect.
you have also to consider that these bastards running labs just to make money will never buy new and expensive machines, the reason we have them in italy it is because they are public healthcare not thought to make money

if it was for private labs they are the same in italy too, no hbsag quantification and even no new hbvdna in the private labs.my sister did hbvdna in a private lab once and she got a wrong result and the limit of quantifccation was 2000iu/ml just riduculous
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Avatar_m_tn
so:) probably i think i cant take that test. coz when they not known abt abbott architect then how would they make manual dilution. very bad,
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Avatar_m_tn

now i see you are in india...they don t find it in US, canada, UK so i think india almost impossible to find,

i think all the communtiy should search for updated labs in US and Asia where to ship blood samples for hbsag quantifications, and of course these labs do exsist

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Avatar_m_tn
yes. i'm going to google it, k, inform us bro if u know,
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Avatar_m_tn
hong kong, singapore, china, japan are the most advanced in asia
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Avatar_m_tn
thank you bro.. but with hbvdna alone cant we do anything?
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Avatar_m_tn
i googled something in a search-hbsag quantification test in india. what is serum hbsag(quantification)? and also tell me the test name for finding hbsag quantity?
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Avatar_m_tn
i googled something in a search-hbsag quantification test in india. what is serum hbsag(quantification)? and also tell me the test name for finding hbsag quantity?
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Avatar_m_tn
no hbvdna has no meaning in terms of infected cells/hbv clearance

and to understand if ntz is working hbsag is essential, the main function of ntz is lower antigens, i think hbvdna lowering is a reasult of antigens lowering:

Rossignol/Gabriella Santoro univ.Torvergata (rome):
Thiazolides have been shown to possess antiviral activity
against two different RNA viruses: hepatitis C, a positive
strand RNA virus, and rotavirus, a double-stranded RNA
virus, and a DNA virus, the hepatitis B virus (36 –39). The
wide spectrum antiviral activity suggests a cell-mediated
effect rather than a specific viral target. The possibility that
maturation of viral glycoproteins may be involved in the
antiviral activity against hepatitis B and C viruses is currently
under study. In the case of rotavirus, TIZ-induced modification
of the structural viral glycoprotein VP7 has been
recently shown,4 reinforcing the hypothesis that maturation
and transport of key viral glycoproteins could be a general
mechanism of the antiviral activity of this new class of drugs.
The finding that thiazolides do not significantly affect the
replication of human rhinovirus, a picornavirus whose maturation
does not require viral glycoprotein trafficking to the
cell membrane, further supports this hypothesis.
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Avatar_m_tn

you should start looking for the biggest universities/hospitals in india where they make research i guess that's the first place
i found a very updated lab in hog kong once making a global search for cccdna quantitication in the blood
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Avatar_m_tn
i have found the names of other assays in a study
HBsAg was measured mainly using the Abbott Architect assay (56%), Roche MODULAR assay (19%), Roche Elecsys HBsAg II assay (8%) or other unspecified assays (12%)

i found this lab but don tknow if it is india
http://www.citypathlabs.com/index.html

their test is: HBsAg (Quantification),serum by CLIA
i don t know about clia but if unit is in iu/ml it is ok
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Avatar_m_tn
happy:-). me too noticed that. that lab is here in our capital only. what matters here is have to know about that clia method.
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Avatar_m_tn
found it. chemiluminescene immunoassay(clia) in abbott website. hope hbsag quantification tests by this method ll be useful.
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Avatar_m_tn
i think it is better to contact the lab and ask them directly how they get hbsag quantitative and which unit

they always answer yes yes and then they make a wrong test after you pay, this has happened to many member, so do ask them the format of the result or just a sample print of a result
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Avatar_m_tn
you can contact dr lalpath labs for HBSAG-quantitative test
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