Perfect , you have done much .
During quantification can some viruses be hidden inside cells so as not be detected:
Any assay that uses blood sample can only measure what is in the blood. HBV "virus" exists in the interior of infected liver cells in 3 forms:
1. cccDNA in the nucleus
2. hbvdna inside HBcAg core protein capsid in the cyctoplasm
3. Complete virion on the way to be released from the cell.
Do all the virus swim in the blood streams or some usually remain in the cells:
HBV virus swim in the blood until they encounter liver cells which they will enter, they can also be engulfed by immune cells such as macrophages and dendritic cells.
When we measure HBsAg in the blood, we are measuring
1. HBsAg in the form of sub-viral particles
2. HBsAg on the surface of circulating HBV virions.
The above are just my simplified descriptions and will not be 100% accurate. To get a better picture, you should do your own study.
retest for simple hbsag kit.its not possible 3 tests neg and one positive. there must b test error. hbsag quant measures only the virus in blood pool not in liver cells, but its value gives an estimate to the extent the liver cells might be infected