Hepatitis C Community
HCV RNA, QUANTITATIVE REAL TIME PCR <43 IU/mL Normal
About This Community:

This forum is for questions about medical issues and research aspects of Hepatitis C such as, questions about being newly diagnosed, questions about current treatments, information and participation in discussions about research studies and clinical trials related to Hepatitis. If you would like to communicate with other people who have been touched by Hepatitis, please visit our new Hepatitis Social/Living with Hepatitis forum

Font Size:
A
A
A
Background:
Blank
Blank
Blank
Blank Blank

HCV RNA, QUANTITATIVE REAL TIME PCR &lt;43 IU/mL Normal

I recently had a test done to verify a False Positive and this is what it reads on the test. HCV RNA, QUANTITATIVE REAL TIME PCR <43 IU/mL Normal  

Can I assume this means it's ok? What does this mean?
Blank
96938_tn?1189803458
You are OK. The result means that no virus was found in the blood sample.  You do not have Hepatitis C
Related Discussions
21 Comments Post a Comment
Blank
96938_tn?1189803458
You are OK. The result means that no virus was found in the blood sample.  You do not have Hepatitis C
Blank
142841_tn?1201978652
Are either of the words "DETECTED" or "UNDETECTED" present on your lab report?
Blank
1491755_tn?1333204962
It means no virus was found at a level greater than 43 copied per ml of blood.  There is NO test that measures to 0 for HCV.

But this test is what 90% of us use to call ourselves undetectable.
Blank
142841_tn?1201978652
Many of the tests that report a lower detection limit of 50, include a qualitative test as well.  So...for instance, you may get a result of 5000, DETECTED.

You might also get a result of <43, UNDETECTED -- which, as you say, is truly virus-free.

You might also receive, as I did, a result of <43, DETECTED.  It seems confusing, until you realize that the quant part of the test is telling you it's below the detection limit, but the qual. part of the test is still detecting low level viremia.

During my last tx, I got to <43 UNDETECTED after 32 weeks of daily Infergen+1400mg Rib.  The next five monthly tests reported as <43 DETECTED.

This represented a viral breakthrough.  I stopped tx, and my viral load shot up in the 10**7 range within a few weeks.

So...it all depends on what test was run to determine whether this is an accurate picture.
Blank
Avatar_n_tn
many thanks for sharing that - the significance of persistent low level VL has been an open question,  particularly since most published data still uses  a lower limit of 50.

Was  the reporting lab Labcorp ? The Roche Diagnostics assay used for that test provides a VL number above 43 but only detected/undetected  status between 15 and 43. Labcorp correctly reports  the result  but some other labs that use the same assay only report  LT 43 with no detected/undetected status. Good luck with your  next round..
Blank
979080_tn?1323437239
Quest Diagnostics only reports LT 43 with no detected/undetected status when you run their HCV RNA Real Time PCR <43.

Blank
720656_tn?1311043835
My lab reports "detected" or "not detected" and the sensitivity was < 25.  I am unsure if this difference is just the way the each lab may report or what the difference may be. I know we all speak to UND but none of my reports stated undetected.
Blank
979080_tn?1323437239
What is the name of your lab ?
Blank
142841_tn?1201978652
Low level viremia is a b*tch, plain and simple.  It is still a positive test,  The tests I had done were proprietary at Stanford, so I don't know if the assay is the same or similar to the Labcorp or other tests.

By the way, RT-PCR is an acronym for Reverse Transcriptase - Polymerase Chain Reaction, not Real Time PCR.
Blank
Avatar_m_tn
The lab that Gee and I used in our trial was the taqman 2.0 here are the lower limits of the test:

"Plasma HCV RNA levels were measured with the use of the TaqMan 2.0 assay (Roche Diagnostics), which has lower limits of quantification and detection of 25 and 9.3 IU per milliliter, respectively; the lower limit of detection was used for decision making at various points throughout the study."
Blank
979080_tn?1323437239
I know the test I am just wondering which lab does it ?
Blank
Avatar_m_tn
My und labs say: HCV RNA not detected, it doesn't say where the lab is.

It comes to our trial investigators from Merck and does not say where the lab is.

Blank
Avatar_n_tn
Hi, what does HBV, quantitative real time PCR value 1153062 IU/ML mean for a person?
Blank
Avatar_n_tn
i just got my results too but i have what seems to be results of 2 types of tests,one says <43 iu/ml but then the other says 2,715,370,what means what?
Blank
1669790_tn?1333666195
Instead of posting your question on an old thread, you would get better results by posting a new question.  

Is this your iniitial test to check your viral load or a 4 week???
Blank
Avatar_n_tn
no,i finished the 48 week treatment in 2009 and was undetectable,i relapsed in 2010 and just came home from a year in prison and all they gave me was the test results but did not explain them,i am trying to understand them to see if i am still considered undetectable
Blank
Avatar_m_tn
It's difficult for us to know what your viral load is without seeing copies of your tests since you have conflicting information. What is the name and date of each test?
-Dave
Blank
Avatar_m_tn
I would say the test is indicating the level of detection at below 43 IU/ml.  Most likely the quantifiable range of the assay is 43 IU/mL to 69,000,000 IU/mL.

I'm not aware of and HCV RNA Quantitative PCR that has a quantifiable range up to 2,715,370, it's a rather odd number but very typical for PCR results if HCV RNA is present so in my opinion that would be the numeric viral load count reported by the assay.

It's would be prudent to have a current PCR run, that way you''ll know exactly what's going on.

Blank
Avatar_m_tn
Correction:

test is indicating the level of quantification at 43 IU/ml or above.  In other words the test can only assign a numerica number is HCV RNA 43 IU/mL or above

Blank
Avatar_m_tn
IF SOME ONE PLZ. CAN TELL ME THE MEANING OF THIS INTERPRETATION OF (HCV PCR(QUANT))

""THE TITER OF HCV IN BLOOD FLUCTUATES IN ACCORDANCE WITH VIRUS LATENCY AND SOMETIME IT MAY BE ABSENT OR BELOW THE SENSITIVITY LIMIT OF THE ASSAY   (VALUE: 3696572 COPIES/ML)
Blank
Avatar_m_tn
Hi ..You have posted your question at the end of an old thread. You will get more responses by going to the orange "post a question " area at the top of the page and starting your own.

If you have a copy of your lab report and post exactly what it says it would be easier for us to interpret,however at first glance it looks like  there is Hepatitis C virus present ,quantified at approx. 3.7 mill  .

Will
Blank
Post a Comment
To
Blank
Weight Tracker
Weight Tracker
Start Tracking Now
Hepatitis C Community Resources
RSS Expert Activity
233488_tn?1310696703
Blank
New Cannabis Article from NORTH Mag...
Jul 20 by John C Hagan III, MD, FACS, FAAOBlank
242532_tn?1269553979
Blank
3 Reasons Why You are Still Binge E...
Jul 14 by Roger Gould, M.D.Blank
242532_tn?1269553979
Blank
Emotional Eating: What Your Closet ...
Jul 09 by Roger Gould, M.D.Blank
Top Hepatitis Answerers
Avatar_f_tn
Blank
dontworry_behappy1
163305_tn?1333672171
Blank
orphanedhawk
Rural Mural, CA
Avatar_f_tn
Blank
nan535
Brooklyn, NY
446474_tn?1404424777
Blank
HectorSF
CA
1815939_tn?1377995399
Blank
pooh55811
96938_tn?1189803458
Blank
FlGuy
South, FL