Generally a reading less than 1:80 (1 part plasma with 8 parts diluting solution (fluid)) is read as a negative ANA.
1:80 is considered a "low positive" and is not confirmatory of any disease. More tests are usually required to confirm the diagnosis. Most of people with 1:80 ANA do not have SLE or any other autoimmune disorder. However all results have to be clinically co-related. So if there are symptoms of an autoimmune disorder at 1:80, then this can be taken as confirmatory for disease.
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