Klonopin works well for the first 8 or so hours after you take it. So if you take it twice a day, you are getting the benefit for 16 hours or so. If you take one .10 in the morning, you will get that benefit for roughly 8 hours only. Klonopin does build up in your system when taken regularly over time. You should take it as your doctor prescribed it to you which I'm assuming is twice a day.