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BAC contig

Hello Dr.

If you are diagnosing a genetic disease and you have genetic sequence with the use of contigs but want to find out it's orientation on a specific chromosome, what is the best procedure that can be used to orient the contig.  Thank you very much.
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If I understand your question correctly you are asking about the chromosomal location of a particular mutated genetic sequence?  Genetic mutations/changes in specific genes are found in specific chromsomes at specific chromsome sites.

To see if the genetic change or mutated genetic sequence is found at the correct chromosomal region, a fluorsescence technique is used to localize the mutated gene sequence on a particular chromosome site.  Using a complementary gene sequence containing a fluorescent tag that is applied to a spread of contracted chromosome under a fluorescent microscope, the correct chromosome should be illuminated at the particular site where the gene is known to be found.  This technique is called FISH, and it is used in molecular genetics to find the chromosome locations of specific genes.
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