please comment on these results, i think that monthly hbsag quant may confirm if we are having a good result from ezetimibe, we have only one thing certain from 2009 to 2012 hbsag stable from 4200 to 7300 no matter what we tried (alinia or imiquimod)
would you also add intf to this combo tdf+etv+ezetimibe since imiquimod too failed?liver specialists scheduled intf add on for this year
ezetimibe is a drug that lowers cholesterol level, isn't it?
I also noticed that decrease in cholesterol affects hbsAg quantity. Since Oct'12 to Dec'12 I lost 3 kilos and
cholesterol went down from 5.6 to 4.95.
HDL was stable around 1.4 but
LDL went down from 4.1 to 3.0
HbsAg went down from 1795 iU/ml to 1447 iU/ml
I did not take any drugs except for the supplements VitD3 1k a day, LivOn GSH+VITC+AntiAge, changed diet a bit.
it may be just a coincidence or improved immune response by those supplements because me and some other members tried simvastatin or red yeast rice supplements in the past and it did lowered chol but no effect on hbsag at all
ezetimibe doesn t work on cholesterol to interfere with hbv but it is thought to interfere with some steps after hbv entry, something before cccdna and hbsag formation.in vitro it did lower cccdna and hbsag but as regards vivo no data yet
as regards hcv ezetimibe blocks cellular entry receptors
I wonder if HbsAg level will stay if you stop ezetimibe...
This looks like a real response that has shifted the daily kinetics of reinfection so that the total cccDNA might be reduced. You took a very high dose, i suspect the regular low dose of 10mg would not have shown much effect.
Since ezetimibe is reported to block virion uptake at a later step it might work synergistic with Myrcludex in reducing the reinfection rate.
The key question is now if a further reduction of surface antigen can be expected, or if this is new setpoint reached with no more progress in infected cell reduction. I think there is a chance that the reduction might continue, similar to what we expect from a properly dosed Myrcludex treatment.
If you see a further drop in upcoming tests, I would definitely recommend to have it as a component add on to the planned IFN therapy. If there is a continuing drop i would not start the IFN as long as the effect shows true progression.
When is your next hbsag quant scheduled?
i can do it whatever timepoint now even with no prescription, it is just 7-10€ cost test and can also try higher dosages since no sides in case of furthur decline
what would you suggest monthly test or every 15 days?
I think we need more time points to make a scientific decision but the result is promising.
I can't find any data on toxicology or dosage escalation studies on Ezitimibe, therefore, I will advice not to exceed 50 mg daily dosage because all the trial with Ezitimibe was 10 mg daily .. This is just my opinion and our great asset Studyforhope can contribute.
Once monthly testing should give meaningful results. However right mow a retesting in two weeks would be useful to confirm that it was not just a temporary fluctuation in surface antigen expression activity without a true reduction in cccDNA content.
I agree that dose increases have to be handled very carefully. Stefano already jumped into uncharted lands with the 50mg dose. I would recommend a test of pancreatic Lipase.
On the other hand, he might not have seen an effect with the standard 10mg dose. It is further possible that he is still in the middle of the dose response curve.
What has to be kept in mind is that this secondary entry inhibitor effect cannot lead directly to an elimination of infected cells. The elimination is depending on ongoing immune mediated infected cell removal or noncytolytic clearance. Once entry blocking is complete, any further dose escalation will achieve nothing but trouble.
But we dont know if the 50mg dose is already causing a near max effect.
is pancreatic lipase urgent?if not i ll test in 2 weeks with hbsag quant
I have seen article on Ezitimibe viral activities and there is a patent in the USA blocking the use of it and the like group on treatment of HBV.
There's possibility that 50mg is still ok but it has to be monitored.
Although this drug is designed for liver metabolism, i ll suggest that due to massive increase in the usual recommended daily dosage to do a Kidney function test if its available.
A daily dosage of 50 mg might well be within the tolerable range and the decision to use 10mg for therapeutic effect is design for long term use.
I am watching closely.
50mg / day seamce to be maximum considered in overdoses. Be careful on the dosage.
"In clinical studies, administration of ezetimibe, 50 mg/day to 15 healthy subjects for up to 14 days, 40 mg/day to 18 patients with primary hyperlipidemia for up to 56 days, and 40 mg/day to 27 patients with homozygous sitosterolemia for 26 weeks was generally well tolerated. One female patient with homozygous sitosterolemia took an accidental overdose of ezetimibe 120 mg/day for 28 days with no reported clinical or laboratory adverse events.
In the event of an overdose, symptomatic and supportive measures should be employed"
creatinine-calcium is monitored every month since i started nucs in 2009 and just rechecked after the 50mg dose adding liver function, no change
lipase is the only test i never did, so i ll keep monthly monitoring of all main tests plus lipase
we have two human trials that covered the 50mg dose and the 40mg dose with no toxicity/sides but they were short time, about 1-2 months only
One female patient with homozygous sitosterolemia took an accidental overdose of ezetimibe 120 mg/day for 28 days with no reported clinical or laboratory adverse events.
can you link this?it may be good to see tests of this patient, anyway i ll keep 50mg for now and if we see a real continuous decrease will consider higher dose with every 4 weeks full monitoring
sorry got the link rxlist.com, good to know no toxicity reported until now
I will look more deeper for the details if published.
No the lipase is not urgent, but important if you proceed. Regarding kidney function, a more sensitive test for kidney toxicity than creatinine will randum urine microalbumine to creatinine ratio. It will pick up minor disturbances to the glomerular podocytes, the most sensitive and sophisticated cells in the kidney.
The ed50 for ezetimibe in the hepaRG cells was 18 micromols. If you remember we calculated once the achievable concentration in a human assuming even distribution in the extracellular fluid. It came out too low. But since ezetimibe is preferentially taken up in the liver, it is possible that much higher local concentrations can be achieved.
The most likely mechanism operating is that a membrane component protein that binds to cholesterol in the HBV envelope after the virions have been endocytosed but not yet fused with the endosome membrane for core delivery into the cytosol is blocked by ezetimibe, blocking envelope to membrane fusion and cytosolic core delivery. The nonfused virion is later destroyed in the endolysosome that will form. A similar mechanism is envisioned for myrcludex, except that in this case the binding of the pres1 arms to the bile acid transporter is blocked.
Thus it can be reasonably expected that these two independent mechanisms to prevent core uptake into the cytosol will work synergistically and could greatly enhance each others efficiency.
A couple questions for you:
1. You stated your ALT dropped from 45-50 down to 30. How long was your ALT that high. Shouldn't it have normalized with ETV + TDF?
2. I read that Ezetimibe also causes hepatitis and increased ALT/AST?
3. I also read that this is sometimes prescribed with Sivastatin. Is there a reason you chose one over the other?
Lastly I wanted to say you should be careful when trying these things, especially when increasing dosing.
1 since 3 months after starting etv.no normalization of ast/alt doesn t happen if there is some immune response
2 no it is not ezetimibe it is the statins added to ezetimibe and in both cases the increase of alt is similar to placebo and due to the dicrease of cholesterol, there are many posts about statins and alt increase.alt normalize continuing statins and does not reflect liver damage
3 that combo is only for those needing to lower cholesterol.the patent over the use of ezetimibe for hbv, hcv recommeds not to combo with statins and to combo with intf
i have full blodd tests plus researchers monitoring, plus we already know 40-50mg dose has no sides for 26weeks so nothing new, the doses over this or for longer periods are something new.
hopefully researchers are trying higher doses too bad they didn t check if there is any toxicity at 100mg or more....the 10mg dose was choosen because there is no incresed effect on cholesterol over this dose, not because of toxicity, and this is bad for us
is it useful to activate macrophages by gcmaf to remove hbsag from circulation faster or it can be deleterious now?
i have some left that will expire anyway so i may use it
What you really want right now is the removal of infected cells faster than the reinfection rate. Ezetimibe holds the chance of effective entry inhibition, if this is the mechanism operating right now that has reduced yor surface antigen level, then you want to truly know that.
If you activate macrophage removal of surface antigen by phagocytosis increase, then you would possibly not be clear if there is indeed the critical effect of ezetimibe at work.
If the effect on cccDNA is real, it will likely continue, once you are sure of that, you can use GCMAF, whatever help it might offer. But better not blur the picture right now, if your 50mg ezetimibe treatment is able to block reinfection sufficiently to reduce cccDNA by unreplenished attrition, it would be an incredible step forward.
Here is a short statement from a paper investigating ezetimibes effect on bile cholesterol in hamsters, but the statement from the discussion relates to dogs. I show it here, because it might contribute to the question of ezetimibe toxicity.
". Despite this, treatment of dogs with ezetimibe at a very high dose (300 mg·day−1·kg body wt−1) for 1 yr did not result in gallstone formation or any other adverse hepatobiliary effects.It should be noted that these findings were made in dogs maintained on a low-cholesterol, low-fat commercial canine diet."
Obviously, using any dose higher than the approved 10mg/day in humans is experimental and has to be done with great caution, under physician supervision and consent and with extensive monitoring of risk indicators. But it is comforting to know that these dogs tolerated such a gigantic dose for a year.