vitd25oh levels Suppresses Hepatitis C not 1.25ohd

[stef2011]

http://onlinelibrary.wiley.com/doi/10.1002/hep.25763/pdf

this is extremely interesting although limitations of study on hcv because it may confirm what we are doing with hbv and vitd25oh levels.too bad nobody is studying this on hbv in vitro and we have to try and see directly ourselves

the higher vitd25oh the stronger the anti assembly effect on hcv

[stef2011]

and should i be supervised by a doctor ?

no if you take 10.000iu daily or less.even better if you avoid dairies because they are not good for health.but you need to check vitd25oh and parathormone every 3 months just to be sure you are getting results.kinetics of vit d are different for every person

[melcul]

Once i get to uk i will start taking vit d too, what blood test results should you monitor in this case ?and should i be supervised by a doctor ?

[mer971]

I found out that i have hep b on January 2013,a routine test blood. Pain begun on February, and only browsing news here I found the importance of Vit D,so on my own i have begun to take it at march 2014. So for 14 months i had pain on my right side,back and forth non stop, and after 1-2 months taking vit d suddenly no more pain, it looks a dream, is that possible? i am living a nightmare,but now that i don't feel it at least i feel more relief. I would suggest it to everyone, at least is a vitamin it's not an antibiotic,nothing to lose.

[stef2011]

yes we dont know on hbv because there are no studies but i dont think there is any mutation in case of hbv

hcv has a very very high mutation rate not comparable to hbv for resistance

also effect of vit d might be different on hbv, i think vit d effect can be more host immune system related than virus

the mice study is very clear in suggesting vit d might have a very strong effect on firbosis/cirrhosis prevention, we have many studies on this but i remember only the mice study because the effect was surprising.

[val_hope]

Mer971,how many months u have been taking 10,000 iu of vit d3 before u noticed that the pain you used to feel in your liver has disappeared?

[yibok]

Hi Stef

Very good article ,

findings suggest that the
treatment of hepatitis C with 25(OH)D3 alone may
not be recommended because of a limited antiviral
effect and emergence of the resistant mutant. However,
if it is combined with compounds that inhibit the
HCV replication such as IFN or protease inhibitors, it
should optimize the antiviral effect while minimizing
the genesis of the mutant.

Does it mean that if we go above a cerain level of 25(OH)D3 , we may resistant mutant over a long period of time . We really dont know what happens with HBV.

[stef2011]

no this is not a coincidence because vit d lowers inflammation and fibrosis

it is long time that we posted this study: they made cirrhosis on mices and made 2 arms.one placebo and one vit d, vit d arm only had mild fibrosis while the other arm had cirrhosis

antiviral effect is probably very mild but anti-cirrhotic is strong

[mer971]

it is a coincidence or it is vit D, from my experience i have to say that I had liver pain,since in march i have taking vit d 10,000, I don't feel pain anymore

[stef2011]

also levels of vitamin d 25oh are important, they say they used in vitro levels 10 times normal levels of general population which is about the levels of the coimbra protocol although they say vitd25oh might be more concentrated in the liver than in general circulation since the liver makes main conversion to 1.25oh

from my experience the maximum levels with no effect on calcium are the best to boost peginterferon therapy, while vit d used monotherapy has no effect at all

[stef2011]

and it also describes hcv resistance mutants to vitd25oh by 32 days treatment in vitro, i have no idea if this can apply to hbv too but hcv is more prone to resistance to everything including interferon itslef than hbv

[stef2011]

it also describes in vitro effects of peginterferon plus vitamin d3

[stef2011]

this is abstract but i strongly suggest to read full article in the link
http://onlinelibrary.wiley.com/doi/10.1002/hep.25763/pdf

25-Hydroxyvitamin D3 suppresses hepatitis C virus production.
Matsumura T1, Kato T, Sugiyama N, Tasaka-Fujita M, Murayama A, Masaki T, Wakita T, Imawari M.
Author information
Abstract
Because the current interferon (IFN)-based treatment for hepatitis C virus (HCV) infection has a therapeutic limitation and side effects, a more efficient therapeutic strategy is desired. Recent studies show that supplementation of vitamin D significantly improves sustained viral response via IFN-based therapy. However, mechanisms and an active molecular form of vitamin D for its anti-HCV effects have not been fully clarified. To address these questions, we infected HuH-7 cells with cell culture-generated HCV in the presence or absence of vitamin D(3) or its metabolites. To our surprise, 25-hydroxyvitamin D(3) [25(OH)D(3) ], but not vitamin D(3) or 1,25-dihydroxyvitamin D(3) , reduced the extra- and intracellular levels of HCV core antigen in a concentration-dependent manner. Single-cycle virus production assay with a CD81-negative cell line reveals that the inhibitory effect of 25(OH)D(3) is at the level of infectious virus assembly but not entry or replication. Long-term 25(OH)D(3) treatment generates a HCV mutant with acquired resistance to 25(OH)D(3) , and this mutation resulting in a N1279Y substitution in the nonstructural region 3 helicase domain is responsible for the resistance.
CONCLUSION:
25(OH)D(3) is a novel anti-HCV agent that targets an infectious viral particle assembly step. This finding provides insight into the improved efficacy of anti-HCV treatment via the combination of vitamin D(3) and IFN. Our results also suggest that 25(OH)D(3) , not vitamin D(3) , is a better therapeutic option in patients with hepatic dysfunction and reduced enzymatic activity for generation of 25(OH)D(3) .
Copyright © 2012 American Association for the Study of Liver Diseases.