Aa
Aa
A
A
A
Close
Avatar universal

SVR persons:Persistence of HCV After Successful Treatment of Chronic Hepatitis C: Is HCV Infection for Life?

Persistence of HCV After Successful Treatment of Chronic Hepatitis C: Is HCV Infection for Life?  

It is presumed that resolution of hepatitis C reflects virus eradication, as evidenced by normalization of liver function tests and disappearance of hepatitis C virus (HCV) RNA from serum, as determined by conventional laboratory assays.

In this study, researchers in Poland examined the expression of the HCV genome in the sera, peripheral blood mononuclear cells (PBMC), and, on some occasions, monocyte-derived dendritic cells (DC) long after resolution of hepatitis C by using a highly sensitive reverse transcription (RT)-polymerase chain reaction-nucleic acid hybridization (RT-PCR-NAH) assay.

The samples obtained from 16 randomly selected patients (5 with spontaneous resolution and 11 with treatment-induced resolution), monitored for up to 5 years, were studied by qualitative and semi-quantitative RT-PCR-NAH and by real-time RT-PCR to detect the HCV RNA positive strand.

The replicative HCV RNA negative strand was examined in PBMC after culture with a T cell proliferation stimulating mitogen.

Results

The findings show that HCV RNA was carried in the convalescent-phase sera and/or PBMC in all 16 individuals investigated.

Also, DC from six of seven patients were reactive for the HCV genome. Importantly, traces of the HCV RNA negative strand, suggesting progressing virus replication, were detected in the majority of mitogen-stimulated PBMC, including four samples collected 5 years after recovery.

Sequencing of the HCV 5' untranslated region fragment revealed genotype 1b in four of nine individuals examined and genotypes 1a and 2a in three and two patients, respectively.

In conclusion, these results imply that HCV RNA can persist at very low levels in the serum and peripheral lymphoid cells and that an intermediate replicative form of the HCV genome can persist in PBMC for many years after apparently complete spontaneous or antiviral therapy-induced resolution of chronic hepatitis C.

Warsaw Medical Academy, Poland.
25 Responses
Sort by: Helpful Oldest Newest
Avatar universal
mike

is there anyway to put those reports in
simple laymens terms

i dont think i could read those on a good day
let alone with this dam brain fog i now have

thanks
ron
Helpful - 0
Avatar universal
Occult hepatits C virus persistence:

identification and characteristics

By Tram N.Q. Pham, PhD, and Tomasz I. Michalak, MD, PhD

Hepatitis C virus (HCV) is a single-stranded RNA virus

of the Flaviviridae family that replicates by making the

so-called
Helpful - 0
137025 tn?1217764741
I think you have found the ultimate cure for spammers.  My hat off to you, I tried being bitchy, being reasonable, being sarcastic, but your approach works very well.  Isolate and overwhelm, I love it.

Good day's work, dude.

Willow
Helpful - 0
Avatar universal
BRL
Your good.

I appreciate you.
Helpful - 0
Avatar universal
Thank you both. I had to eat or I could have gone on and on. I don't like the board junked up with the same stuff over and over again so I thought I'd add some of my stuff - I have a ton of it. Mike
Helpful - 0
Avatar universal
Statins Slow Lung Function Decline in Smokers

Martha Kerr
Information from Industry
NSCLC Treatment StrategiesFor 1st-line chemotherapy treatment, learn more about GEMZAR
Helpful - 0
Have an Answer?

You are reading content posted in the Hepatitis C Community

Top Hepatitis Answerers
317787 tn?1473358451
DC
683231 tn?1467323017
Auburn, WA
Learn About Top Answerers
Didn't find the answer you were looking for?
Ask a question
Answer a few simple questions about your Hep C treatment journey.

Those who qualify may receive up to $100 for their time.
Explore More In Our Hep C Learning Center
image description
Learn about this treatable virus.
image description
Getting tested for this viral infection.
image description
3 key steps to getting on treatment.
image description
4 steps to getting on therapy.
image description
What you need to know about Hep C drugs.
image description
How the drugs might affect you.
image description
These tips may up your chances of a cure.
Popular Resources
A list of national and international resources and hotlines to help connect you to needed health and medical services.
Herpes sores blister, then burst, scab and heal.
Herpes spreads by oral, vaginal and anal sex.
STIs are the most common cause of genital sores.
Condoms are the most effective way to prevent HIV and STDs.
PrEP is used by people with high risk to prevent HIV infection.