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pregnancy test help!!!!

I didn't have any issues with pregnancy test when I found out but a good friend of mine has some questions so I figured I'd ask. I took my pregnancy test in the morning when I woke up, but if you take it during the day could it make difference between a pos and neg result?
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If you're very early on, you might test positive first thing in the morning and have a negative later on. First morning urine is more concentrated and therefore Hcg levels will be higher and more detectable than in the more dilute urine passed later in the day.
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