i dont think so because sperm dies off very quickly
When you ejaculate during masterbation there is still a small amount of sperm in the urethra. So there will be sprem mixed in your pre-***. Having unprotected sex in that short period of time will increase her chances of getting pregnant greatly, even if you don't ejaculate. Use protection or wait a couple days before having unprotected sex (after you ejaculate) to minimize the chance of pregnancy. Please remember though, there will always be a risk of pregnancy when you have unprotected sex.
All you need is one sperm, and men have billions, no matter how much you masterbate and release, there is always new sperm created.
If you had sex unprocted yes. Even more so if you ejaculated in her. Even if you didnt there is still a possiblity if you didnt use a condom