Hi all. The doctors forum is full at the moment - but I am hoping there are a few experts out there who can answer my question. I am coming to the end of an IVF cycle - blood test on monday - I already have taken a couple of tests and they are negative. I have no symptoms of pregnancy either - just an impending period - so I am expecting the worst on Monday. ICSI was used as my husbands sperm count is low. Last June 12 months the count was 30 mil, with about 15 mil mobile - which was low but not too bad. Before christmas another test was done and it was less than 1 mill. When he gave his sample for the IVF two weeks ago it was less than 100,000 and he was asked to give a second sample (God help him). We got 2 good embryos which were transferred. I have just turned 42 a week ago. My husband is the same age. He smokes - about 8 rollup cigarettes a day, drinks a couple of glasses of wine about 4 nights a week. He works on computers all day, is reasonably fit - goes running at weekends for about an hour. What is the cause of the rapid decline in sperm count and quality? Is there anything he / we can do. I am so anxious that this IVF cycle is our last chance at having a baby that we long for. He went for a checkup about 8 months ago because he was feeling discomfort or a tightness in his testicles. He had an ultrasound but they found nothing. They prescribed antibiotics as a precautionary measure. CAn anyone shed any light? Thank you.