Bands found, or tested for, in IgG analysis in USA or Mexico.
18-kDa
20-kDa
21-kDa
22-kDa
23-kDa
24-kDa
28-kDa
29-kDa
30-kDa
31-kDa
34-kDa
35-kDa
39-kDa
41-kDa
45-kDa
55-kDa
58-kDa
88-kDa
62-kDa
66-kDa
93-kDa
Notes:
A serum sample was considered positive by WB [IgG] if at least three of
the following protein bands were recognized: 18, 24, 28, 29, 31, 34, 39,
41, 45, 58, 62, 66, and 93 kDa. PMID: 10071428
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=10071428&form=6&db=m&Dopt=b
93, 39, 34 or 23 kDa IgG bands - PMID: 9580180
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9580180&form=6&db=m&Dopt=b
55-kDa - PMID: 7642278
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=7642278&form=6&db=m&Dopt=b
The recommended criteria for a positive IgG immunoblot are the
recognition of two of five proteins (20, 24 [> 19 intensity units], 35,
39, and 88 kDa). Alternatively, if band intensity cannot be measured,
the 22-kDa protein can be substituted for the 24-kDa protein with only
a small decrease in sensitivity. PMID: 7714202
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=7714202&form=6&db=m&Dopt=b
at least 5 of the 10 most frequent IgG bands after the first weeks of
infection (18, 21, 28, 30, 39, 41, 45, 58, 66, and 93 kDa)-PMID: 8380611
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8380611&form=6&db=m&Dopt=b
66 kDa and 31/34 kDa - PMID: 3819479
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=3819479&form=6&db=m&Dopt=b
-----
Other bands found in IgM/IgG in other countries:
Europe/Germany - PMID: 9163458
The following interpretation criteria resulting in specificities of
greater than 96% were recommended: for IgG WB, at least one band of
p83/100, p58, p56, OspC, p21, and p17a for PKa2; at least two
bands of p83/100, p58, p43, p39, p30, OspC, p21, p17, and p14 for
PKo; and at least one band of p83/100, p39, OspC, p21, and p17b
for PBi; for IgM WB, at least one band of p39, OspC, and p17a or
a strong p41 band for PKa2; at least one band of p39, OspC, and
p17 or a strong p41 band for PKo; and at least one band of p39 and
OspC or a strong p41 band for PBi.
IgG titers to P35 and P37 Mice/Northern blot - PMID: 9175831
Italy - PMID: 8809552
The overall reactivity of the
three Borrelia strains in IgG immunoblots indicated that ten
protein bands were significant, with a different prevalence of some
of them in the two groups of patient sera: bands at 60-58, 30-33,
36-37 and 28-27 kDa were markers for neuroborreliosis sera;
proteins at 100-83, 72-70 and 18-17 kDa behaved like markers for
Lyme arthritis. The IgM Immunoblots revealed significant bands
at 100-83, 72-70, 51, 24- 21 and 18-17 kDa only with
neuroborreliosis sera.
Russia - PMID: 7791165
IgG reactivity with > or = 5 spirochetal proteins, particularly the
37, 39, 41, 60, and 93 kDa antigens
Germany - PMID: 8167425
IgG - 95 and 19-17 kDa
France - PMID: 8405312
[IgG] Reactions with specific protein bands (94, 73, 30 and 21 KDa)
-----
The "CDC recommended" bands for Western blot testing - CDC/MMWR 1995.
The ASTPHLD, CDC, FDA, NIH, CSTE, NCCLS 1994 conference recommendation
("CDC recommendation") for the serologic diagnosis of Lyme disease -
CDC/MMWR 1995:
ASTPHLD - Association of State and Territorial Public Health Laboratory
Directors
CDC - Centers for Disease Control and Prevention
FDA - Food and Drug Administration
NIH - National Institutes of Health
CSTE - Council of State and Territorial Epidemiologists
NCCLS - National Committee for Clinical Laboratory Standards
According to CDC's Morbidity and Mortality Weekly Report (MMWR) 1995;
44 (31):590-591, an IgM immunoblot is considered positive if two of the
following three bands are present:
24 kDa (OspC)*
39 kDa (BmpA)
41 kDa (Fla)
An IgG immunoblot is considered positive if five of the following 10
bands are present:
18 kDa
21 kDa (OspC)*
28 kDa
30 kDa
39 kDa (BmpA)
41 kDa (Fla)
45 kDa
58 kDa (not GroEL)
66 kDa
93 kDa
* The apparent molecular mass of OspC is dependent on the strain of
B. burgdorferi being tested. The 24 kDa and 21 kDa proteins referred
to are the same.
Recommendations for Test Performance and Interpretation from the Second
National Conference on Serologic Diagnosis of Lyme Disease,
CDC MMWR, August 11, 1995 / 44(31);590-591
http://www.cdc.gov/epo/mmwr/preview/mmwrhtml/00038469.htm
or
http://wonder.cdc.gov/wonder/prevguid/m0038469/entire.htm
-----
For more information about the Western blot test and Lyme disease, see:
The National Lyme Disease Network LymeNet Newsletter
Volume 4 - Number 13 - 9/23/96
SPECIAL ISSUE - Understanding the Western Blot
http://www2.lymenet.org/domino/nl.nsf/UID/4-13
Explanation of the Lyme Disease Western Blot by Carl Brenner
http://www.lymealliance.org/Medical/MedCategory4/Lab4/lab4.html
Laboratory Tests by Tom Grier - Part 2 - Western Blot and ELISA
http://www.lymealliance.org/Medical/MedCategory4/Med12A/med12a.html
IGeneX, Inc. - Lyme Disease Western Blot
http://www.igenex.com/lymeset2.htm
MRL Diagnostics' Lyme Disease B. burgdorferi Genogroup 1
Western Blot IgG test
http://www.mrldiagnostics.com/insert/wb0400g.htm
Immuno-Biological Laboratories (IBL) - Hamburg [Germany] -
Borrelia burgdorferi/Lyme IgG Western Blot
http://www.ibl-hamburg.com/prod/re_97228.htm
The western immunoblot for Lyme disease: determination of sensitivity,
specificity, and interpretive criteria with use of commercially
available performance panels.
Tilton RC, Sand MN, Manak M
BBI Clinical Laboratories, Inc., New Britain, Connecticut 06053, USA.
Clin Infect Dis 1997 Jul;25 Suppl 1:S31-S34
http://www.x-l.net/Lyme/BBI_TEST.htm
SIMULTANEOUS ELISA AND WESTERN BLOT TESTING IN EVALUATION OF PATIENTS
FOR SUSPECTED LYME DISEASE
Janice M. Kochevar, FNP-C, Kenneth B. Liegner, M.D.
LDF 10th Annual International Scientific Conference on Lyme Borreliosis
& Other Tick-borne Disorders: April 28-30, 1997
http://www.x-l.net/Lyme/elisawb497.htm
MEDLINE -
Western Immunoblot*/blot* [in Title] AND Lyme Disease - 33 on 1 Mar 2001
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=PubMed&orig_db=PubMed&dispmax=1000&doptcmdl=DocSum&term=%28western%5BTI%5D+AND+%28immunoblot*%5BTI%5D+OR+blot*%5BTI%5D%29+AND+%28lyme+OR+burgdorferi+OR+%28borreli*+AND+ixodes%29+OR+%28%28erythema+AND+migrans%29+NOT+glossitis%29+OR+garinii+OR+afzelii+OR+neuroborreli*%29
-----
For more information on Lyme disease, see:
Lots Of Links On Lyme Disease
http://www.geocities.com/HotSprings/Oasis/6455/lyme-links.html
-----
Prepared by
A.D.
---
OspA = 29-33.5 kDa
Regarding OspA AND Lyme disease the following molecular weights are
associated with OspA in the MEDLINE database:
Note: To see the abstract for a particular PMID number,
simply insert the number, via copy and paste, in the following URL
after the "&list_uids=" term:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=&dopt=Abstract
Example, using the first number below:
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10638032&dopt=Abstract
29-31 kDa - PMID: 10638032, 8429231
29.6 kDa - PMID: 1406458
30 kDa - PMID: 7500914
30-32 kDa - PMID: 2461135
31-kDa - PMID: 10030131, PMID: 8005219, PMID: 8406878, PMID: 1520966
PMID: 1554741, PMID: 10699329, PMID: 8825913
32-kDa - PMID: 8005219, PMID: 1520966
32-kDa/rOspA dog vaccine - PMID: 8567917
32.5 kDa - PMID: 9144917, PMID: 8004045
33 kDa - PMID: 1520966, PMID: 8005219
33.5 kDa - PMID: 8004045, PMID: 1520966
---
OspB 34-kDa PMID: 10030131
OspC 21-25-kDa, 22-25-kDa - PMID: 8825913,
OspD 28-kDa, PMID: 8825913
OspE 19.2-kDa [calculated], PMID: 8262642
OspF 26.1-kDa [calculated], PMID: 8262642
-----
Bands specific for Borrelia burgdorferi:
14-kDa ?
21-kDa
22-kDa OspC
25-kDa OspC
28-kDa OspD
31-kDa OspA
34-kDa OspB
35-kDa
37-kDa
39-kDa
47-kDa
50-kDa
83-kDa
93-kDa
94-kDa
Notes:
14-kDa ? PMID: 9920119, PMID: 1556546
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9920119&form=6&db=m&Dopt=b
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=1556546&form=6&db=m&Dopt=b
B. burgdorferi: 22 kDa, 31 kDa, 34 kDa, 39 kDa, 83 kDa - PMID: 9440203
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9440203&form=6&db=m&Dopt=b
p35 and p37 are Borrelia burgdorferi genes - PMID: 9175831
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9175831&form=6&db=m&Dopt=b
at least one is an apparently specific band (25, 28, 39, 47, 50, or
93 kDa). - PMID: 7791177
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=7791177&form=6&db=m&Dopt=b
antibodies against the 94 kDa, 31 kDa and 21 kDa proteins are largely
species-specific. - PMID: 8223404
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8223404&form=6&db=m&Dopt=b
-----
Cross-reacting bands:
19-kDa
22-kDa ??
20-kDa
30-kDa
33-kDa
34-kDa ??
36-kDa
40-kDa
41-kDa
60-kDa
66-kDa
72-kDa
Notes:
Search terms:
cross-react*
cross-antigenicity
crossreact
Immunoblot analysis indicated the presence of cross-reacting antibodies
directed to B. burgdorferi antigens with apparent molecular weights of
60, 41, 40, 36, 30 and 20 kDa. - PMID: 1385332
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=&form=6&db=m&Dopt=b
P66, P60, P41 which are dominant immunogens of all types of borrelias
PMID: 9162453
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9162453&form=6&db=m&Dopt=b
19, 22[??], 72 - PMID: 1372635
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=1372635&form=6&db=m&Dopt=b
60-75 kDa range, p40, p33 and two proteins in the range of 20 kDa. -
PMID: 1597198
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=1597198&form=6&db=m&Dopt=b
Whereas the 60 kDa, 41 kDa, and 34 kDa[??] constituents reveal a
marked cross-antigenicity with other spirochetes and even more distantly
related bacteria,...PMID: 8223404
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8223404&form=6&db=m&Dopt=b
-----
Bands found, or tested for, in IgM analysis in USA or Mexico.
18-kDa
21-kDa
23-kDa
24-kDa
25-kDa
28-kDa
37-kDa
39-kDa
41-kDa
45-kDa
55-kDa
58-kDa
60-kDa
66-kDa
93-kDa
Notes:
37 - PMID: 9986810
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9986810&form=6&db=m&Dopt=b
41 kDa and 58 kDa - PMID: 9972057
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9972057&form=6&db=m&Dopt=b
24 kDa (OspC), 41 kDa, and 37 kDa - PMID: 8748261
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8748261&form=6&db=m&Dopt=b
39, 58, 60, 66, or 93 kDa - PMID: 8748261
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8748261&form=6&db=m&Dopt=b
55-kDa - PMID: 7642278
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=7642278&form=6&db=m&Dopt=b
Recommended criteria for the immunoglobulin M (IgM) immunoblot are the
recognition of two of three proteins (24, 39, and 41 kDa). PMID: 7714202
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=7714202&form=6&db=m&Dopt=b
25-kDa antigens - PMID: 8308100
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8308100&form=6&db=m&Dopt=b
23-kDa - PMID: 8225587
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8225587&form=6&db=m&Dopt=b
at least 2 of the 8 most common IgM bands in early disease
(18, 21, 28, 37, 41, 45, 58, and 93 kDa) - PMID: 8380611
http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8380611&form=6&db=m&Dopt=b
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