Generally speaking, yes it is. But this also depends on if you actually hold your urine all night long and then test, or if you're up every few hours or such.
Pee first thing in the morning! So thats & hours of no peeing lol
Its really just the longest you hold your pee is when its best to test. It was always best for me to test in the afternoon because I' didn't have time at work to pee.
Just thought it was starnge that not peeing for 7hours gets a lighter line
then holding it for 2 hours lol!