Hi all. I am a teacher in South Africa. One of my passions and hobbies is to try and educate the youth regarding STD's and prevention thereof. I am familiarising myself with STD transmission risks and other information, however from time to time a varsity student tends to ask me something that simply has me at loss for a decent educated answer. Recently I bought a box of rapid HIV test kits (blood/plasma) so that I could have a discussion and testing session with a few first year students who admitted to sometimes taking sexual risks. Everyone enjoyed the experience and pledged to always practice safe sex after our discussion and testing exercise. I am happy to report that no positive test results came out of the day (15 students tested); however I do not view HIV as being a death sentence or something that anyone should ever stimatize others over! I made it very clear to the students that although safety and prevention are extremely important, people living with HIV are just like anyone else and should never be treated otherwise. Anyhow, after we were done, one student came and asked me something that scared me a bit since I did not know the answer and I allowed the students to pull apart their testing devices (pricked fingers exposed to the device) to see what was inside etc. before the question was asked. The question was if HIV antigen was not the main ingredient of the testing strip and if so, there was not a risk of acquiring HIV from the testing kit itself. I simply did not know the answer and all I could say in response is that I am sure the kits would not be sold to the public if there was any such risk. I am now quite petrified that I might have exposed all those young people to a possible risk. Many let their pipettes touch the testing pad when they transferred blood into the device and then drew more blood from their fingers to add (as we used various YouTube videos as a guide and most everyone who tested on the videos had done the same thing). Others (my students) pulled apart their devices after testing to see what was inside and further proceeded to dissect the inner workings of their tests with their exposed pre-pricked fingers. I left them as the whole exercise amused them greatly and I thought it served as a good learning experience. Even I dissected my test in the same fashion like a total idiot! I came home and started researching the topic, however all I can gather from the internet and one other post I found on this site (regarding an oral test) is that a dormant form of antigen is used, however there was not much info on the subject. Is this correct? Most information I can find is regarding the oral versions of testing kits and not really the blood testing kits. Does anyone know scientifically how these kits work and if there is any risk at all? I can't honestly see how as they are sold commercially with no warnings regarding this, but then again in this day and age you never know! Also, is there any risk of being exposed to the buffer liquid, since I have no idea what is inside that either. I am looking for fact/scientific based answers and not assumptions, since I want to inform the students next week regarding the answers I receive. All answers I receive will be greatly appreciated. P.S sorry this is so long :-)