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HBV genotyping test, DNA limit of 1000iu/ml is it true?
I was told by the lab that they can not run the genotyping test, because my viral load is too low (21 iu/ml). They said the minimum of 1000iu/ml is required.
Is it specific to the equipment and I can find another lab with different analyzer or it is the general rule?
Is it specific to the equipment and I can find another lab with different analyzer or it is the general rule?
hbeag neg has 0% hbsag clearance on antivirals or so, less than clearance naturally which is about 3% per year
the only way to clear on hbeag neg is pegninterferon or nuc long time and pegintf add on
on tenofovir hbeag pos geno A and D are almost the only ones clearing hbsag in about 16% at 3 years
same thing for etv but percentage is about 5% only
i think it is important to know because:
mixed genotypes have increased risk of hcc/cirrhosis and less response to treatment
geno C is at increased risk for hcc
geno a can clear much easier on intf
Thank you Stef! I will certainly include Pisa to my next trip to Italy.
With my viral load 21 ui/ml the genotype is not much important, but anyway I want to know as much as possible about my virus.
With my viral load 21 ui/ml the genotype is not much important, but anyway I want to know as much as possible about my virus.
if you move to italy you can do it, we dont have distinction between research or commercial, you can have it in pisa where i did the tests, it is an int'l reserch center and they have patients from all over the world since it is well known how advanced they are, if you have ryanair flights close it can be very cheap
Just have got the feed back from the lab, they confirmed that the technique you have mentioned is only used in Research environment. They have no approval for using this protocol in "mass production" testing.
Thank you for the help anyway!
Thank you for the help anyway!
research in italy is applied directly to patients, i mean scientists are often also clinician doctors, so if you choose one of these university hospitals you have all commercial and not commercial tests available, they dont waste the machines just for research since they are all paid by public healthcare (so paid by us)
direct sequence by nested pcr of small hbsag by use of "dye terminator" and "elettroforesi capillare" by CEQ 8000
cant translate this because i dont know what it is:"elettroforesi capillare" google translates "capillary electrophoresis"
If it is not a problem to you, please check.
This lab is Cebra, it is one of the biggest in France, and I am in contact with the chief infectionist from there.
By the way the research centre you are talking about is their lab open for public? I come to Italy several times a year, maybe it will be a chance to get tested there.
Have a great rest of the day!
This lab is Cebra, it is one of the biggest in France, and I am in contact with the chief infectionist from there.
By the way the research centre you are talking about is their lab open for public? I come to Italy several times a year, maybe it will be a chance to get tested there.
Have a great rest of the day!
problem is i had tests in a research lab so they have all the best among commercial machines/kits, if you are not in europe you may have a hard time to find these tests
if you really need it i can check tomorrow
Thank you Stef.
Can you provide any supportive reference so I could convince the Lab?
I found another lab which uses Roche kit, and they also said that if DNA is less than 1000 the result is unreliable.
Can you provide any supportive reference so I could convince the Lab?
I found another lab which uses Roche kit, and they also said that if DNA is less than 1000 the result is unreliable.
hbvdna pcr according to test sensibility can quantificate hbvdna upto 20iu/ml or 6iu/ml according to test sensibility, for lower values the pcr can detect the presence of the virus but not quantificate
for example if it says <20iu/ml detected, hbvdna can range from 1iu/ml to 20iu/ml
if it says <20iu/ml not detected, hbvdna is not detactable
of course when you try to detect between 1 and 10iu/ml the test can miss the presence of virus so low and be und even if some is present
anyway hbvdna pcr is not so important today as long as the virus is less than 20iu/ml or possibly less than 6iu/ml, what matters most if hbsag quantity
for example if it says <20iu/ml detected, hbvdna can range from 1iu/ml to 20iu/ml
if it says <20iu/ml not detected, hbvdna is not detactable
of course when you try to detect between 1 and 10iu/ml the test can miss the presence of virus so low and be und even if some is present
anyway hbvdna pcr is not so important today as long as the virus is less than 20iu/ml or possibly less than 6iu/ml, what matters most if hbsag quantity
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resistance test canbe performed even with hbvdna less than 20iu/ml but still detactable hbvdna, they just use pcr two times withvery sensitive tests