Aa
Indeterminate RIBA (reaction to NS-5 band)
I found out that my indeterminate RIBA test was reactive to the NS-5 band, others were all negative...Does anyone know much about the various bands and their significance?....As from a post I made few days ago, ALT, Bilirubin, were normal in the recent lab..I'm doing the PCR test later today...Couldn't find one signifcant risk factor for HCV that is why i was so stunned at the indeterminate finding....
I found this to so it sounds like NS-5 is indeterminate, its significance is based on risk factor of patient, or something like that?...Confusing stuff, that's for sure...
"Isolated C100 reactivity is thought to be less significant than C33c and C22 as marker of a former infection and is often considered non-specific12. Isolated NS-5 reactivity may have different implications. This marker is known to be diagnostically relevant for HCV infection screening in high-risk subjects, because it may accompany viraemia13. In blood donors, such reactivity may have a different significance. In our series, eight out of nine subjects (89%) with isolated reactivity to NS-5 repeatedly tested negative with the HCV-RNA and did not seroconvert throughout the follow-up period. Our data do, therefore, support other authors’ conclusions6,12 that NS-5 reactivity in blood donors is mostly non-specific. Considering that subjects reactive only for NS-5 represent almost half the donors with an indeterminate status (27 out of 65 in the whole cohort, 9 out of 24 of those who completed follow-up), since the introduction of viral RNA amplification testing, the finding of this band in first-level donor selection tests is counterproductive rather than beneficial, causing numerous transfusable blood units to be discarded and expensive testing to be conducted in those donors with an indeterminate HCV status"...
"Isolated C100 reactivity is thought to be less significant than C33c and C22 as marker of a former infection and is often considered non-specific12. Isolated NS-5 reactivity may have different implications. This marker is known to be diagnostically relevant for HCV infection screening in high-risk subjects, because it may accompany viraemia13. In blood donors, such reactivity may have a different significance. In our series, eight out of nine subjects (89%) with isolated reactivity to NS-5 repeatedly tested negative with the HCV-RNA and did not seroconvert throughout the follow-up period. Our data do, therefore, support other authors’ conclusions6,12 that NS-5 reactivity in blood donors is mostly non-specific. Considering that subjects reactive only for NS-5 represent almost half the donors with an indeterminate status (27 out of 65 in the whole cohort, 9 out of 24 of those who completed follow-up), since the introduction of viral RNA amplification testing, the finding of this band in first-level donor selection tests is counterproductive rather than beneficial, causing numerous transfusable blood units to be discarded and expensive testing to be conducted in those donors with an indeterminate HCV status"...
If you go about half way down to page 11 it explains it better than I could... with an "indeterminate riba then the true tell tale test is the one you did today ..the PCR and very often with an inteterminate Riba the end result is a Neg. PCR .
Good luck and let us know...
Will
http://hepatitiscnewdrugs.blogspot.com/search/label/protease%20inhibitor-%20%28NS3%2F4A%29%20Drug%20Resistance%20Test
Good luck and let us know...
Will
http://hepatitiscnewdrugs.blogspot.com/search/label/protease%20inhibitor-%20%28NS3%2F4A%29%20Drug%20Resistance%20Test
Here is the way I understand it...and I will look for data in a few minutes
The Atigen Band pattern goes like this
If no Bands are reactive but the "hsod alone is reactive then the result = Negative
If 1 band is reactive "or" the "hsod and one band is reactive the result= Indeterminate
If 2 or more Bands are reactive then the result - Positive
So..the way I understand your result...just the NS-5 was the only positive....so it is indeterminate.
Let me look for something that can explain ...hopefully better than that
Will
The Atigen Band pattern goes like this
If no Bands are reactive but the "hsod alone is reactive then the result = Negative
If 1 band is reactive "or" the "hsod and one band is reactive the result= Indeterminate
If 2 or more Bands are reactive then the result - Positive
So..the way I understand your result...just the NS-5 was the only positive....so it is indeterminate.
Let me look for something that can explain ...hopefully better than that
Will
hi Will,
Man, im confused on all this, i thought positive on "hsod" meant positive...Then i read something like this, and its more puzzling:
"Among the 31 samples with indeterminate RIBA results, 22 were attributable to isolated antibodies to c33c or NS5 proteins. Damen et al. (30) evaluated a third-generation RIBA in 530 anti-HCV-positive individuals. Only 1 of 220 HCV RNA-positive individuals had indeterminate RIBA, and none had a negative RIBA result. They found only 1 of 55 individuals with isolated antibody to c33c, and none of 51 individuals with isolated antibody to NS5 to be HCV RNA-positive. Overall, 20% of RIBA results in their study had one of these two indeterminate patterns (30). In two other studies, HCV RNA was never detected in individuals with isolated antibody to NS5 on RIBA (31)(32). Although the number in our study was small, we found HCV RNA in only 1 of 12 persons with isolated anti-NS5 or anti-c33c. These data support our conclusion that most low-positive anti-HCV results represent cross-reactive antibodies causing false-positive anti-HCV results".
Man, im confused on all this, i thought positive on "hsod" meant positive...Then i read something like this, and its more puzzling:
"Among the 31 samples with indeterminate RIBA results, 22 were attributable to isolated antibodies to c33c or NS5 proteins. Damen et al. (30) evaluated a third-generation RIBA in 530 anti-HCV-positive individuals. Only 1 of 220 HCV RNA-positive individuals had indeterminate RIBA, and none had a negative RIBA result. They found only 1 of 55 individuals with isolated antibody to c33c, and none of 51 individuals with isolated antibody to NS5 to be HCV RNA-positive. Overall, 20% of RIBA results in their study had one of these two indeterminate patterns (30). In two other studies, HCV RNA was never detected in individuals with isolated antibody to NS5 on RIBA (31)(32). Although the number in our study was small, we found HCV RNA in only 1 of 12 persons with isolated anti-NS5 or anti-c33c. These data support our conclusion that most low-positive anti-HCV results represent cross-reactive antibodies causing false-positive anti-HCV results".
Hi...ok if only one band of the 5 was positive then it still means the Riba test is "indeterminate " If the only band that was positive was the" hsod" band then it would be a negative result
With only the one band positive there is an excellent chance you PCR will be negative and the Riba was a false positive.
Good luck with the PCR test.
Will
With only the one band positive there is an excellent chance you PCR will be negative and the Riba was a false positive.
Good luck with the PCR test.
Will
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