Aa
Interferon with weekly vaccine
Though vaccine is useless for us now it is coming in my mind y not take it weekly along with my interferon shots. Can it make any difference? Has this thing ever studied on immune change. Any idea or previous study? We have done several self experiments so can this be tried as well but some info on this if any?
thank you so much for your inputs, if many of these mini trials were possible around the world in countries less regulated, i think that approval would be much easier
i ll be definitely using extremely good professionals, not normal nurse for sure.
i am sure dr brunetto or the team can help with the microrna signature to understand if we are moving to sustained immunity
if we are going to do this combination we will make it at best possible level or best not to do it
i am sure dr brunetto or the team can help with the microrna signature to understand if we are moving to sustained immunity
if we are going to do this combination we will make it at best possible level or best not to do it
You would need to know many details, best learned with a doctor or nurse. like you have to use a 250 0r 500ml bag of NaCl for the long infusions, you need an infusion pump with the bag that you can program, a sterility 0.22 micron in line filter and an iv catheter, not a needle for these long infusions. If the liquid goes para it could cause very bad inflammations in the tissue there, with the nonmethylated ac. You must never try s c injections.
If you use the methylated form for one hour you can use a syringe pump, but still an in line sterility and particle filter. None of this can be recommended for a layperson, as simple as it is in the end once everything is worked out. It would be really best if your Pisa docs would at least introduce you first to all the proper handling.
Most importantly, before starting anything practical, you must understand what the real mechanisms are that would lead to a permanent success and how to guide and steer towards this goal.
using just the best nap is unlikely to permanently seroconvert more than 10 to 20% of patients, probably less if e neg and if they are in your position with multiple flares and the resulting epitope burnout, it is even much harder to achieve that. The combo with Zadaxin is a must, but if it does not give you a high antibody titer after say 4 month, you need to add the cuban vaccine.
Once you do ha e a high antibody titer, there is still no reason to celebrate. Look at the outcome of trial number 2. Now comes the time to understand what is truly going on, why does the ab disappear so quickly once the treatment is stopped? What is the mechanical role of the ab in the long term stabilisation of the remnant infected cell number?.
If you use the methylated form for one hour you can use a syringe pump, but still an in line sterility and particle filter. None of this can be recommended for a layperson, as simple as it is in the end once everything is worked out. It would be really best if your Pisa docs would at least introduce you first to all the proper handling.
Most importantly, before starting anything practical, you must understand what the real mechanisms are that would lead to a permanent success and how to guide and steer towards this goal.
using just the best nap is unlikely to permanently seroconvert more than 10 to 20% of patients, probably less if e neg and if they are in your position with multiple flares and the resulting epitope burnout, it is even much harder to achieve that. The combo with Zadaxin is a must, but if it does not give you a high antibody titer after say 4 month, you need to add the cuban vaccine.
Once you do ha e a high antibody titer, there is still no reason to celebrate. Look at the outcome of trial number 2. Now comes the time to understand what is truly going on, why does the ab disappear so quickly once the treatment is stopped? What is the mechanical role of the ab in the long term stabilisation of the remnant infected cell number?.
Infusion time of 10hrs not a problem at all once a week
if we already know these naps have no sides effects requiring day hospital it will be no problem
pisa will do monitoring only like it was for gcmaf, i dont think they can use naps
if we already know these naps have no sides effects requiring day hospital it will be no problem
pisa will do monitoring only like it was for gcmaf, i dont think they can use naps
potency of (ac)20 DNA phophorothio is better than 2139, but very long infusion time ( 6 hours for 200 mg, 8 to 10 hours for 400mg) is needed and some immune activating effects will be present, although no comparison to interferon itself. 5 methylating all the twenty deoxyCytosins will improve potency by a factor of 2 and side effects, in particular if presaturated with Magnesium chloride, will be almost eliminated. Infusion time one to two hours is optimum, not shorter, or you will loose too much into the urine. I wonder if the Pisa researchers would give you the green light for this.
And Most important thing, rep2055 less complicated and expensive so showing formula to genscript might get us the compound
i think we can also make up a small group to do this and a group of researchers, hopefully somebody from pisa, so genscript might be more interested
i think we can also make up a small group to do this and a group of researchers, hopefully somebody from pisa, so genscript might be more interested
So the cheapest and most simple is rep 2055 which can still be good at potency/sides effect vs cost in our combo?
as to cost we are in the range of 5000-15000euro for 48weeks?
To me best combo would be:
vit d, gcmaf, naps, zadaxin, cuban vaccine, tdf
tdf for additiona 6months/year even after stable hbsab
as to cost we are in the range of 5000-15000euro for 48weeks?
To me best combo would be:
vit d, gcmaf, naps, zadaxin, cuban vaccine, tdf
tdf for additiona 6months/year even after stable hbsab
I don't think they would respond positively to this request. These oligos are for lab use only. You are talking about RNA (AC) 20 with all 2 prime positions modified with a methoxy group (that is O-CH3) and all Cytosin bases having a methyl group on their 5 position. Fully phosphorothioate of course, for the phosphate bonds.
As I said, very expensive modifications, twice the dose needed.
As I said, very expensive modifications, twice the dose needed.
This company is just one out of many, that will produce custom oligos.
If you look at all the possible modifications it is indeed confusing certainly to a layperson. The wrong oligonucleotides could indeed be toxic. But the nap used in the first trial was very simple.single stranded dna oligo.fully phosphorothioate linkage. Twenty ACs in a string, no other modifications. Delivered as sodium salt. It needs to be infused very slowly, 200mg in 500ml of normal saline in 6 hours.the cytidine exerts a cpg like effect on toll like 9 receptors, that is the main reason. It also grabs divalent ions like calcium in the plasma, that's why a presaturation with ca or mg will eliminate this effect. One ml of a one molar magnesium chloride solution added to the infusion will work to achieve that. To eliminate the cpg effect, the cytidine can have a methyl group in the 5 position of the base. This dramatically improves tolerability and increases the hbsag suppressive effect about twofold. This would constitute the ideal nap. Don't know why replicor never tried this one. But there is no question, the company tries their best to find the best suited nap, but is hampered by the slow and rigid nature of these trials.
If you look at all the possible modifications it is indeed confusing certainly to a layperson. The wrong oligonucleotides could indeed be toxic. But the nap used in the first trial was very simple.single stranded dna oligo.fully phosphorothioate linkage. Twenty ACs in a string, no other modifications. Delivered as sodium salt. It needs to be infused very slowly, 200mg in 500ml of normal saline in 6 hours.the cytidine exerts a cpg like effect on toll like 9 receptors, that is the main reason. It also grabs divalent ions like calcium in the plasma, that's why a presaturation with ca or mg will eliminate this effect. One ml of a one molar magnesium chloride solution added to the infusion will work to achieve that. To eliminate the cpg effect, the cytidine can have a methyl group in the 5 position of the base. This dramatically improves tolerability and increases the hbsag suppressive effect about twofold. This would constitute the ideal nap. Don't know why replicor never tried this one. But there is no question, the company tries their best to find the best suited nap, but is hampered by the slow and rigid nature of these trials.
https://drive.google.com/file/d/0B8E77QizhkLQNS05Vng1WExxNVk/view?usp=sharing
what if we go to http://www.genscript.com/italy/gene_synthesis.html# and ask we want the compound rep 2139 and show the file in the link, do they understand what we want?
i am afraid we are all close to zero knowledge in molecular biology or even simple chemist
what if we go to http://www.genscript.com/italy/gene_synthesis.html# and ask we want the compound rep 2139 and show the file in the link, do they understand what we want?
i am afraid we are all close to zero knowledge in molecular biology or even simple chemist
http://replicor.com/wp-content/uploads/2015/04/Replicor-NAPS-vs-DHBV-in-vivo-EASL-2015-P0542-FINAL.pdf
compounds are here 2139 i think is the best because the one used in latest trials, but also price is important
Tomorrow I'll be taking next weekly vaccine inj with IFN. Did not read much about oligonucleotides in past few days due to busy schedule. Will request if any of u cud add some more of ur knowledge on this. As I see their are a a number of different oligonucleotides, it is a matter of confusion which one is the most appropriate for hbv and closest to replicor compounds. I am sort of layman on this topic so fearing a bit to self experiment without any prior documentation. How toxic these cud be is wat the big question. So before I say one shall try this it might be better to get near to the most right compound as much possible.
I am watching the following website, if relevant do add some views and also share more relevant site links on appropriate oligos.
I am watching the following website, if relevant do add some views and also share more relevant site links on appropriate oligos.
up, i think the purpose of meronabebe spamming is to hide useful posts or just a psyco, in any case i bring up our open interesting and most useful posts
The answer to your question is given if you study the fine details of trial number 2. You will see that thymosin alpha is more effective than interferon.
But thymosin is not approved in us or europe, these have to be the target markets.
But thymosin is not approved in us or europe, these have to be the target markets.
The prices for oligonucleotides vary strongly for different modifications. The 2139 needs 500MG weekly and costs twice as much as the simple (ac) 20 without modifications per gram. So the final cost for a year are 5 times higher.
The ca or mg are just added at the time of the infusion. So it would be the difference between for example 15000 euros and 75000.
All formulas are shown on the website.
The ca or mg are just added at the time of the infusion. So it would be the difference between for example 15000 euros and 75000.
All formulas are shown on the website.
If one has enough money he has over 65% chance of "cure" from Hep B.
I wonder why did they use combo of Repac + Peg-Inf instead of Repac + Zadaxin in the latest clinical triall...was it just for regulatory purposes or do they deem it to be more effective long term solution....
I wonder why did they use combo of Repac + Peg-Inf instead of Repac + Zadaxin in the latest clinical triall...was it just for regulatory purposes or do they deem it to be more effective long term solution....
what are the extact details (forumulas, amount etc..) a person needs to know if want to order synthesis for example rep 2139ca ?
Better get some info on it March then try it. I've read few things on it and got messed up. So many types which one two choose.. What are the methods to use them, their biochemical details, infusion, titration etc. don't u c Studyforhope is also saying know all risks careful study and infusions n all. Give some time then go for it. Also share here something if u get useful. Also it'll be beneficial only in combo not mono.
Ohh that's some fearful to know. I am little scared with that coz have no idea what it cud do on my body.
Normal infusions of antibiotics and IV fluids I know but this thing seeming strange. Alright I'll search for it on net now and tell u what Info I get.
Normal infusions of antibiotics and IV fluids I know but this thing seeming strange. Alright I'll search for it on net now and tell u what Info I get.
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