I am sorry to hear about your medical problems. High ASO titre is of extreme importance in <15 years age group and are a marker of streptococcal infection. Group A Streptococcus is responsible for diseases such as glomerulonephritis, rheumatic fever (causes joint pains), bacterial endocarditis, and scarlet fever. The ASO level is usually less than 160 Todd units per milliliter. In your case the titer is high. In adults this has no significance unless there are specific symptoms related to streptococcal infection like sore throat, skin infections, heart symptoms or kidney symptoms. The titre will go down with penidure for sometime but this treatment is generally not required. I think you should not worry too much about your ASO titres. Get a complete heart and kidney checkup. If everything is fine do not worry.
Generally a reading less than 1:80 (1 part plasma with 8 parts diluting solution (fluid)) is read as a negative ANA.
1:80 is considered a "low positive" and is not confirmatory of any disease. More tests are usually required to confirm the diagnosis. Most of people with 1:80 ANA do not have SLE or any other autoimmune disorder. However all results have to be clinically co-related. So if there are symptoms of an autoimmune disorder at 1:80, then this can be taken as confirmatory for disease. Since you have joint pain, complete antibody analysis along with rheumatoid factor (RF) analysis will need to be performed.
There is no conclusive test to diagnose rheumatoid arthritis (RA). If you have joint pains then the possibility is looked into by doing a RF and X-rays. Also many patients with RA have normal RF and many with high RF do not have RA.
Please discuss in detail with your doctor. Take care!
The medical advice given should not be considered a substitute for medical care provided by a doctor who can examine you. The advice may not be completely correct for you as the doctor cannot examine you and does not know your complete medical history. Hence this reply to your post should only be considered as a guiding line and you must consult your doctor at the earliest for your medical problem.