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Are there any medications or therapies which INACTIVATES THE HBV cccDNA
I would just like to know if anyone here in the community have any research which relates to the approaches in which the cccDNA can be blocked/destroyed/inactivated/interfered since from some of the forums here the culprit to the persistence of the virions and the surface antigen would be this cccDNA template inside the hepatocyte? is it in the cytoplasmic area or in the nucleus of the liver cell?
Have you found out any herbal medicines or any drugs which can block this template? please share your research regarding the cccDNA. thanks.
Have you found out any herbal medicines or any drugs which can block this template? please share your research regarding the cccDNA. thanks.
nope, chronic, confirmed 1/2009 (VL over 10 million) and again 3/2009 (VL 300 thousand)
It was the tea, it was all I use during that time period when viral load was reduce 97%
Even on pharmasuiticals or if I was not chronic, that much load would not clear on its own.
I'm Confident enough that people will research this and find the same promising data and have the same results.
What is questionable is your repeated use of the word scam. It is like it’s your design to undermine my validity and to intelligent people that brings op the question of your motive.
You are sounding just like Big Pharma.
Are you?
It was the tea, it was all I use during that time period when viral load was reduce 97%
Even on pharmasuiticals or if I was not chronic, that much load would not clear on its own.
I'm Confident enough that people will research this and find the same promising data and have the same results.
What is questionable is your repeated use of the word scam. It is like it’s your design to undermine my validity and to intelligent people that brings op the question of your motive.
You are sounding just like Big Pharma.
Are you?
it is not the tea it is immune clearance phase from immune tollerant phase, everyone of us has had it, if it was tea you'd clear hbeag and hbsag too
if you were not cronic it is even more normal more than 95% adults clear hbv
your post is just pure scam....within a very serious thread
I had a VL of over 10 Million and reduced it to just 300k in 2 months by drinking a tea called Chanca Piedra from sunfood.com
Please google this stuff. You will really have to dig deep online to find that one of the scientists who discovered the surface antigen for HB wrote this stuff could clear chronic HB.
I am now down to a VL of 252 hundred and it is nearly gone. I stopped using the tea a while ago and switched to taking yeast bound selenium 200 mcg 2 times a day, a multivitamin containing Alpha Lipoic Acid and 2000 mg of vitamin D.
I also take 500 mg of Olive leaf powder and Astragalus rood caps.
All of the above are anti viral but in different ways. Astragalus activates the body’s natural interferon response, Alpha Lipoic Acid is an anti viral but also protects the liver from damage, Olive leaf is antiviral that prevents viruses from replicating.
Please google this stuff. You will really have to dig deep online to find that one of the scientists who discovered the surface antigen for HB wrote this stuff could clear chronic HB.
I am now down to a VL of 252 hundred and it is nearly gone. I stopped using the tea a while ago and switched to taking yeast bound selenium 200 mcg 2 times a day, a multivitamin containing Alpha Lipoic Acid and 2000 mg of vitamin D.
I also take 500 mg of Olive leaf powder and Astragalus rood caps.
All of the above are anti viral but in different ways. Astragalus activates the body’s natural interferon response, Alpha Lipoic Acid is an anti viral but also protects the liver from damage, Olive leaf is antiviral that prevents viruses from replicating.
alinia (ntz) does most of this by activating PKR and eIf 2alpha phospholyration, it activates by cascade tnf aplha,ctl and so on
https://docs.google.com/fileview?id=0B_yFgxI8KNcRMGU1NTRmNTctMzU3MS00MjMzLTk3YzMtNDFhNWZmNzBkNjc4&hl=en
of course if ntz is active on bacteria so different, viruses so diffent, even some cancers in the stomach, the effect is clearly on immune system.
the problem is still the dose of the drug which is problematic if more than 2g on the stomach.it is very clear that higher doses have higher response
another problem is the virus suppressing pkr because research has proven almost all viruses suppress pkr
https://docs.google.com/fileview?id=0B_yFgxI8KNcRMGU1NTRmNTctMzU3MS00MjMzLTk3YzMtNDFhNWZmNzBkNjc4&hl=en
of course if ntz is active on bacteria so different, viruses so diffent, even some cancers in the stomach, the effect is clearly on immune system.
the problem is still the dose of the drug which is problematic if more than 2g on the stomach.it is very clear that higher doses have higher response
another problem is the virus suppressing pkr because research has proven almost all viruses suppress pkr
based on the previous article (Intracellular Inactivation of the Hepatitis B Virus by Cytotoxic T Lymphocytes) it seems that TNF alpha and Interferon gamma may play the noncytolytic T cell mediated pathway in clearing the virus.
i am currently researching on drugs or herbs which may potentiate this two important cytokines for viral clearance.
another article i have seen which we might be able to apply involves FORSKOLIN. information from wikipedia on forskolin: Forskolin (also called Coleonol) is a labdane diterpene that is produced by the Indian Coleus plant (Coleus forskohlii).
Hild, Marc et al. Glucagon Treatment Interferes with an Early Step of Duck Hepatitis B Virus Infection. J Virol, April 1998, p. 2600-2606, Vol. 72, No. 4.
"The effect of glucagon on the establishment of hepadnavirus infection was studied in vitro with the duck hepatitis B virus (DHBV) model. The presence of the peptide hormone throughout infection or starting up to 8 h after virus uptake resulted in a dose-dependent reduction in the levels of intra- and extracellular viral gene products and of secreted virions. Treatment with forskolin or dibutyryl-cyclic AMP, two drugs that also stimulate the cyclic AMP (cAMP) signal transduction pathway, resulted in comparable inhibition, suggesting that the inhibitor effect is related to changes in the activity of protein kinase A. In persistently infected hepatocytes, only a slight, but continuous, decrease in viral replication was observed upon prolonged drug treatment."
i am currently researching on drugs or herbs which may potentiate this two important cytokines for viral clearance.
another article i have seen which we might be able to apply involves FORSKOLIN. information from wikipedia on forskolin: Forskolin (also called Coleonol) is a labdane diterpene that is produced by the Indian Coleus plant (Coleus forskohlii).
Hild, Marc et al. Glucagon Treatment Interferes with an Early Step of Duck Hepatitis B Virus Infection. J Virol, April 1998, p. 2600-2606, Vol. 72, No. 4.
"The effect of glucagon on the establishment of hepadnavirus infection was studied in vitro with the duck hepatitis B virus (DHBV) model. The presence of the peptide hormone throughout infection or starting up to 8 h after virus uptake resulted in a dose-dependent reduction in the levels of intra- and extracellular viral gene products and of secreted virions. Treatment with forskolin or dibutyryl-cyclic AMP, two drugs that also stimulate the cyclic AMP (cAMP) signal transduction pathway, resulted in comparable inhibition, suggesting that the inhibitor effect is related to changes in the activity of protein kinase A. In persistently infected hepatocytes, only a slight, but continuous, decrease in viral replication was observed upon prolonged drug treatment."
i have found an interesting article. just want to post it just for future reference.
Guidotti, Luca G et al. Intracellular Inactivation of the Hepatitis B Virus by Cytotoxic T Lymphocytes. Immunity, Volume 4, Issue 1, 25-36, 1 January 1996. (available at http://www.cell.com/immunity/retrieve/pii/S1074761300802952)
Important excerpts from the article:
"...we also demonstrate that the antiviral effect of the CTL is mediated by at least two inflammatory cytokines and DOES NOT MERELY REPRESENT THE DESTRUCTION OF HBsAg POSITIVE HEPATOCYTES. There are several reasons why we believe these claims are valid.
1. clearance of viral nucleocapsids and replicative DNA intermediates begins prior to the onset of liver disease, and eventually involves every hepatocyte in these animals.
2. using wild-type CTL the disease is relatively mild, consisting of only small sparsely scattered necroinflammatory foci that kill a very small fraction of the hepatocytes, while all traces of the virus (except the integrated transgene) disappear from virtually 100% of the hepatocytes.
3. the cytopathic and regulatory effects of the CTL can be dissociated either by the administration of antibodies to IFNγ and TNFα, which completely abrogate the antiviral effect without reducing disease severity, or by administration of HBsAg-specific perforinless CTL, which completely abrogate HBV replication without causing liver disease.
... The first detectable effect of the CTL on viral replication is the disappearance of cytoplasmic viral nucleocapsids and replicative DNA intermediates from the liver.
... While the authors emphasized the importance of hepatocellular regeneration for viral clearance in these studies, inflammation and macrophage (Kupffer cell) activation may have also contributed to this effect. In an independent study, the same investigators demonstrated that WHV clearance for the woodchuck liver is MOSTLY INDEPENDENT of hepatocellular destruction and regeneration during massive acute WHV infection ( [20]), suggesting that other factors might play an important role in viral clearance in that model. It is noteworthy that in the present experiments, HBV gene expression and replication were completely abolished following the transfer of cytokine-producing perforinless CTL.
...Absolute clearance requires elimination of the episomal covalently closed circular (ccc) HBV DNA species that serves as the viral transcriptional template in the nucleus of .. since ccc DNA is abolished in the massive WHV infection model described above in the absence of massive destruction or regeneration of hepatocytes and in the presence of inflammatory cells and Kupffer cell hyperplasia, it is likely that ccc DNA can indeed be abolished from the resting hepatocyte by cytokine-dependent pathways...
Guidotti, Luca G et al. Intracellular Inactivation of the Hepatitis B Virus by Cytotoxic T Lymphocytes. Immunity, Volume 4, Issue 1, 25-36, 1 January 1996. (available at http://www.cell.com/immunity/retrieve/pii/S1074761300802952)
Important excerpts from the article:
"...we also demonstrate that the antiviral effect of the CTL is mediated by at least two inflammatory cytokines and DOES NOT MERELY REPRESENT THE DESTRUCTION OF HBsAg POSITIVE HEPATOCYTES. There are several reasons why we believe these claims are valid.
1. clearance of viral nucleocapsids and replicative DNA intermediates begins prior to the onset of liver disease, and eventually involves every hepatocyte in these animals.
2. using wild-type CTL the disease is relatively mild, consisting of only small sparsely scattered necroinflammatory foci that kill a very small fraction of the hepatocytes, while all traces of the virus (except the integrated transgene) disappear from virtually 100% of the hepatocytes.
3. the cytopathic and regulatory effects of the CTL can be dissociated either by the administration of antibodies to IFNγ and TNFα, which completely abrogate the antiviral effect without reducing disease severity, or by administration of HBsAg-specific perforinless CTL, which completely abrogate HBV replication without causing liver disease.
... The first detectable effect of the CTL on viral replication is the disappearance of cytoplasmic viral nucleocapsids and replicative DNA intermediates from the liver.
... While the authors emphasized the importance of hepatocellular regeneration for viral clearance in these studies, inflammation and macrophage (Kupffer cell) activation may have also contributed to this effect. In an independent study, the same investigators demonstrated that WHV clearance for the woodchuck liver is MOSTLY INDEPENDENT of hepatocellular destruction and regeneration during massive acute WHV infection ( [20]), suggesting that other factors might play an important role in viral clearance in that model. It is noteworthy that in the present experiments, HBV gene expression and replication were completely abolished following the transfer of cytokine-producing perforinless CTL.
...Absolute clearance requires elimination of the episomal covalently closed circular (ccc) HBV DNA species that serves as the viral transcriptional template in the nucleus of .. since ccc DNA is abolished in the massive WHV infection model described above in the absence of massive destruction or regeneration of hepatocytes and in the presence of inflammatory cells and Kupffer cell hyperplasia, it is likely that ccc DNA can indeed be abolished from the resting hepatocyte by cytokine-dependent pathways...
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