Aa
Interferon with weekly vaccine
Though vaccine is useless for us now it is coming in my mind y not take it weekly along with my interferon shots. Can it make any difference? Has this thing ever studied on immune change. Any idea or previous study? We have done several self experiments so can this be tried as well but some info on this if any?
potency of (ac)20 DNA phophorothio is better than 2139, but very long infusion time ( 6 hours for 200 mg, 8 to 10 hours for 400mg) is needed and some immune activating effects will be present, although no comparison to interferon itself. 5 methylating all the twenty deoxyCytosins will improve potency by a factor of 2 and side effects, in particular if presaturated with Magnesium chloride, will be almost eliminated. Infusion time one to two hours is optimum, not shorter, or you will loose too much into the urine. I wonder if the Pisa researchers would give you the green light for this.
And Most important thing, rep2055 less complicated and expensive so showing formula to genscript might get us the compound
i think we can also make up a small group to do this and a group of researchers, hopefully somebody from pisa, so genscript might be more interested
i think we can also make up a small group to do this and a group of researchers, hopefully somebody from pisa, so genscript might be more interested
So the cheapest and most simple is rep 2055 which can still be good at potency/sides effect vs cost in our combo?
as to cost we are in the range of 5000-15000euro for 48weeks?
To me best combo would be:
vit d, gcmaf, naps, zadaxin, cuban vaccine, tdf
tdf for additiona 6months/year even after stable hbsab
as to cost we are in the range of 5000-15000euro for 48weeks?
To me best combo would be:
vit d, gcmaf, naps, zadaxin, cuban vaccine, tdf
tdf for additiona 6months/year even after stable hbsab
I don't think they would respond positively to this request. These oligos are for lab use only. You are talking about RNA (AC) 20 with all 2 prime positions modified with a methoxy group (that is O-CH3) and all Cytosin bases having a methyl group on their 5 position. Fully phosphorothioate of course, for the phosphate bonds.
As I said, very expensive modifications, twice the dose needed.
As I said, very expensive modifications, twice the dose needed.
This company is just one out of many, that will produce custom oligos.
If you look at all the possible modifications it is indeed confusing certainly to a layperson. The wrong oligonucleotides could indeed be toxic. But the nap used in the first trial was very simple.single stranded dna oligo.fully phosphorothioate linkage. Twenty ACs in a string, no other modifications. Delivered as sodium salt. It needs to be infused very slowly, 200mg in 500ml of normal saline in 6 hours.the cytidine exerts a cpg like effect on toll like 9 receptors, that is the main reason. It also grabs divalent ions like calcium in the plasma, that's why a presaturation with ca or mg will eliminate this effect. One ml of a one molar magnesium chloride solution added to the infusion will work to achieve that. To eliminate the cpg effect, the cytidine can have a methyl group in the 5 position of the base. This dramatically improves tolerability and increases the hbsag suppressive effect about twofold. This would constitute the ideal nap. Don't know why replicor never tried this one. But there is no question, the company tries their best to find the best suited nap, but is hampered by the slow and rigid nature of these trials.
If you look at all the possible modifications it is indeed confusing certainly to a layperson. The wrong oligonucleotides could indeed be toxic. But the nap used in the first trial was very simple.single stranded dna oligo.fully phosphorothioate linkage. Twenty ACs in a string, no other modifications. Delivered as sodium salt. It needs to be infused very slowly, 200mg in 500ml of normal saline in 6 hours.the cytidine exerts a cpg like effect on toll like 9 receptors, that is the main reason. It also grabs divalent ions like calcium in the plasma, that's why a presaturation with ca or mg will eliminate this effect. One ml of a one molar magnesium chloride solution added to the infusion will work to achieve that. To eliminate the cpg effect, the cytidine can have a methyl group in the 5 position of the base. This dramatically improves tolerability and increases the hbsag suppressive effect about twofold. This would constitute the ideal nap. Don't know why replicor never tried this one. But there is no question, the company tries their best to find the best suited nap, but is hampered by the slow and rigid nature of these trials.
https://drive.google.com/file/d/0B8E77QizhkLQNS05Vng1WExxNVk/view?usp=sharing
what if we go to http://www.genscript.com/italy/gene_synthesis.html# and ask we want the compound rep 2139 and show the file in the link, do they understand what we want?
i am afraid we are all close to zero knowledge in molecular biology or even simple chemist
what if we go to http://www.genscript.com/italy/gene_synthesis.html# and ask we want the compound rep 2139 and show the file in the link, do they understand what we want?
i am afraid we are all close to zero knowledge in molecular biology or even simple chemist
Have an Answer?
You are reading content posted in the Hepatitis B Community
A list of national and international resources and hotlines to help connect you to needed health and medical services.
Herpes sores blister, then burst, scab and heal.
Herpes spreads by oral, vaginal and anal sex.
STIs are the most common cause of genital sores.
Condoms are the most effective way to prevent HIV and STDs.
PrEP is used by people with high risk to prevent HIV infection.
