Takeing your Sub properly is important for full absorption. Drinking something hot right before is suggested. Keeping it under your tongue until completely disolved (5-20 minutes), no talking, smoking or drinking while you're doing your dose.
I don't think spliting your dose has any effect on max effect, but alot of people do split it so that the effect is more balanced thruout the day.
I don't know the answer to your question, so I'm answering to bring your question back to the top.
Someone will reply that knows the answer.
Friend999