I had a boarder who also had the same problem. Turns out he was very uncomfortable with sharing the washroom with other people. Could possibly be an embarassing situation for your boy, and he might feel he's going to offend his grandparents, and doesn't want to say anything. Another incident that comes to mind, is that through the night some people are shy, and the thought of coming out of the washroom in underwear or pj's through the night could be embarrasing. It's a new situation for him. Possibly once he gets comfortable with his grandparents, and knows they will respect his privacy, the problem will diminish.
Is he able to get to the restroom when he needs to? Don't accept that he doesn't know why at this point. You have to be very empathetic when talking to him, and ask what he is thinking about that makes him do this. Tell him it's okay that he won't be in any trouble, just ask "why do you choose this instead of the bathroom." Ask him if sharing the bathroom with his grandparents is bothering him, and if so it's okay, we''ll work something out. If he still insists that he doesn't know why then I would have him see a professional about this, he may be really mising his brothers and his anxiety is taking this route. I'm just guessing, but if he stops and you see no other odd behavior it may be okay. But I would be very vigilant with his actions, so you can stop something before it gets worse.