Autoimmune Disorders Expert Forum
BAC contig
About This Forum:

Welcome to the Autoimmune Disorders forum. Questions in this forum are being answered by Dr. Garth Nicolson. This forum is for questions and discussions relating to Autoimmune Disorders. Topics include, but are not limited to: Alzheimer’s, Amyotrophic Lateral Sclerosis (ALS), Chronic Fatigue Syndrome, Fibromyalgia, Gulf War Syndrome, Lupus, Lyme Disease, MS, Rheumatoid Arthritis

Please note, Dr. Nicolson is a part-time expert at MedHelp, so this forum will only be open to new questions during pre-arranged dates.

Font Size:
A
A
A
Background:
Blank
Blank
Blank
This expert forum is not accepting new questions. Please post your question in one of our medical support communities.
Blank Blank

BAC contig

Hello Dr.

If you are diagnosing a genetic disease and you have genetic sequence with the use of contigs but want to find out it's orientation on a specific chromosome, what is the best procedure that can be used to orient the contig.  Thank you very much.
Related Discussions
642304_tn?1242610324
If I understand your question correctly you are asking about the chromosomal location of a particular mutated genetic sequence?  Genetic mutations/changes in specific genes are found in specific chromsomes at specific chromsome sites.

To see if the genetic change or mutated genetic sequence is found at the correct chromosomal region, a fluorsescence technique is used to localize the mutated gene sequence on a particular chromosome site.  Using a complementary gene sequence containing a fluorescent tag that is applied to a spread of contracted chromosome under a fluorescent microscope, the correct chromosome should be illuminated at the particular site where the gene is known to be found.  This technique is called FISH, and it is used in molecular genetics to find the chromosome locations of specific genes.
Blank
Continue discussion Blank
MedHelp Health Answers
Blank
Weight Tracker
Weight Tracker
Start Tracking Now
RSS Expert Activity
469720_tn?1388149949
Blank
Abdominal Aortic Aneurysm-treatable... Blank
Oct 04 by Lee Kirksey, MDBlank
242532_tn?1269553979
Blank
The 3 Essentials to Ending Emotiona...
Sep 18 by Roger Gould, M.D.Blank
242532_tn?1269553979
Blank
Control Emotional Eating with this ...
Sep 04 by Roger Gould, M.D.Blank